FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_87.35100000041e31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_87.35100000041e31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118155
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC2024117.130887393677796No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT1841415.584613431509458No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG18691.5818204900342772No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTAC207.011488E-444.994923
TTATACT207.011488E-444.994924
CGCAAAT207.011488E-444.9949212
GCCGTCT207.011488E-444.9949245
TGCCGTC20900.044.56434645
TTTATAC403.4262666E-739.3705523
CGGTGTA250.002096282836.01117329
ACGGTGT250.002096282836.01117328
ATTTACG250.002100645835.9959371
CATATGC250.002100645835.99593719
TACGAAT250.002100645835.9959374
TAGATAA250.002100645835.99593740
GAACAGC250.002100645835.9959376
GGTGTAG300.005121018330.00931230
GAACTAA300.005121018330.00931232
TACGGTG300.005121018330.00931227
ATACTAG300.005121018330.00931225
GTGTAGT300.005121018330.00931231
GGTCTAT300.005121018330.00931231
ATACGGT300.005121018330.00931226
Produced by FastQC (version 0.11.2)