FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_86.35100000041e17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_86.35100000041e17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359834
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC86652.408054825280546No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA42241.17387461996365No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC37161.0326984109339306No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC32890.9140325816904462No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC30950.8601188325727975No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC28030.7789703029730376No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT27810.7728563726607269No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG26910.7478448395649105No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA25770.7161635643102097No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC17140.4763307525136591No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC16650.4627133622726035No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA15730.43714601733021335No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC15140.4207495678562893No Hit
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC13820.38406598598242525No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG13330.3704485957413696No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA13170.36600210096878005No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT12530.3482161218784217No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGT12520.34793821595513486No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG12330.34265800341268476No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACG12330.34265800341268476No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACT10790.29986049122651No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTAC9230.2565071671937616No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC9080.25233857834445883No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTT8490.23594212887053473No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTAC8120.22565960970892135No Hit
CTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGA8020.22288055047605285No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG7990.2220468327061923No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG6420.178415602750157No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCA6290.17480282574742798No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCT6000.16674355397210935No Hit
GTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTAC5950.16535402435567512No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGC5840.16229705919951978No Hit
ATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCA5710.15868428219679073No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATACA5450.15145872819133266No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACA5350.14867966895846418No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC5170.1436773623393009No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC4920.13672971425712968No Hit
CAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGG4750.13200531356125322No Hit
GGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGA4660.1295041602516716No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGC4450.12366813586264779No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT4290.11922164109005819No Hit
GCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTA4060.11282980485446067No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAG3900.10838331008187108No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTA3850.10699378046543685No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA3650.10143566199969985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGGT351.2078999E-745.004313
TGCCGTC3600.040.62324545
GTCGCGC403.4505683E-739.37338
GGTCGCG403.4505683E-739.37337
GCGCGCT800.036.5609211
TTAGTAT4100.034.0276532
TATGCCG4200.033.74854343
ATGCCGT4300.033.48692344
CGTCTTA4250.033.35613628
CGAGACG4400.033.23719823
GTCTTAG4300.032.9682729
CGTATGC4300.032.96368841
GTATGCC4300.032.96368842
TAGTATC4250.032.8266733
TCGTATG4250.032.82211340
TCTTAGT4350.032.58932530
CTCGTAT4300.032.44045639
TATCTCG4250.032.2927236
CTTAGTA4500.032.00306331
AACTGCG501.977287E-631.4986387
Produced by FastQC (version 0.11.2)