FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_85.35100000041dfc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_85.35100000041dfc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604659
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC134332.221582743331365No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT108801.7993612928940115No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG13250.2191317750996843No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC6360.10518325204784845No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC14000.042.9109545
GGGGACA250.00210711935.997227
GCCGTCT401.5592892E-533.75018745
TGTCGGC406.1655184E-428.12283110
CCACGAC709.301566E-725.7123019
ACCCGCT450.001225252225.0022056
CCGGCTG450.001226434324.9980734
CGTGTCG450.001226434324.998078
TACAGCC450.001226434324.998077
ATGCCGT24800.024.22392544
TATGCCG26200.022.92951843
CGTATGC26750.022.5421841
GACGTGT602.4641617E-422.5019846
TTAGACG500.002262218422.5019844
TCGTATG26600.022.50012440
GTATGCC26800.022.50012242
GTGTCGG702.7018667E-522.4982649
CGCGCGC500.00226439422.49826210
CCCGCTG500.00226439422.4982627
CACGACA602.4669222E-422.4982610
Produced by FastQC (version 0.11.2)