FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_80.35100000041d4f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_80.35100000041d4f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34260
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC6591.923525977816696No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT2180.6363105662580268No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG1040.30356100408639813No Hit
AGAAGAAGTAGAAGAAGAAGAAGAAGAAGAAGAGGAAGAAGCAGCAGAAGA410.11967308814944543No Hit
AAGGAAAGGAAAGGAAAGAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAG400.11675423234092236No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAT206.944409E-444.9912429
TGCCGTC751.5861588E-929.99416245
ATTAGAT405.9990824E-428.1606371
AGCCCAC1001.2350938E-924.74518217
GCCCACG1001.2350938E-924.74518218
CGAGCCC1101.382432E-1024.54067615
TCCGAGC1101.382432E-1024.54067613
CCACGAG1052.1755113E-923.5668420
GAGCCCA1052.1755113E-923.5668416
ACGAGAC1052.1755113E-923.5668422
CGAGACT1052.1755113E-923.5668423
CCGAGCC1152.401066E-1023.4736914
CTCCGAG1152.401066E-1023.4736912
CACGAGA1103.7252903E-922.4956221
CCCACGA1103.7252903E-922.4956219
ATGCCGT1003.3978722E-822.49561944
TCTCCGA1204.0927262E-1022.49561911
GAGACTG1156.2245817E-921.5175524
AATCTCG1055.6963472E-821.424436
ACTGATT1055.6963472E-821.424427
Produced by FastQC (version 0.11.2)