FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_8.35100000041448.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_8.35100000041448.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34403
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC32829.53986570938581No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGT3591.043513647065663No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCG1210.35171351335639334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3800.040.84734745
CCACGAG3950.039.8656920
ACAATGC3950.039.8656927
GAGACAA3950.039.8656924
CGTTATC3900.039.7999833
TGCGTTA3900.039.7999831
CGAGCCC4050.039.43680215
CAATGCG4000.039.36736728
GCCCACG4000.039.36736718
GAGCCCA4000.039.36736716
CACGAGA4000.039.36736721
CCCACGA4000.039.36736719
GACAATG4000.039.36736726
CGTATGC4000.039.36736741
CCGAGCC4000.039.36736714
CGAGACA4000.039.36736723
TTATCTC3950.039.2961835
TCTCGTA3950.039.2961838
GCGTTAT3950.039.2961832
GTTATCT3950.039.2961834
Produced by FastQC (version 0.11.2)