FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_79.35100000041d25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_79.35100000041d25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263650
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC230878.756684999051773No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT51951.9704153233453443No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG35461.3449649156078134No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACA10290.39029015740565143No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC9930.37663569125734875No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATACA9670.3667741323724635No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC9400.3565332827612365No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT8830.3349137113597573No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA8690.3296036411909729No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC8380.3178456286743789No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA7760.294329603641191No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATCT7480.28370946330362223No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATC7480.28370946330362223No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA7260.27536506732410393No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC6860.2601934382704343No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATA6690.2537454959226247No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT6590.2499525886592073No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATACACATCTCC6020.22833301725772806No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCCTGTCTCTTATA5980.22681585435236107No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC5820.22074720273089324No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC5580.21164422529869145No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT5460.20709273658259053No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG5410.20519628295088185No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA5320.20178266641380618No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC5230.19836904987673049No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT5200.19723117769770532No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA5180.1964725962450218No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT4790.18168025791769393No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA4640.1759908970225678No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC4450.16878437322207474No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTC4380.16612933813768255No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC4290.16271572160060688No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC4260.16157784942158165No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTGTCTCTTATACA4160.15778494215816424No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT4060.15399203489474683No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT3980.1509577090840129No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA3900.14792338327327897No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC3850.14602692964157027No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG3820.14488905746254505No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACACT3630.13768253366205196No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT3610.13692395220936848No Hit
GATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA3610.13692395220936848No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG3510.13313104494595107No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACACATCTCC3330.12630381187179973No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACA3300.1251659396927745No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACAT3260.12364877678740754No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCAT3240.12289019533472406No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACA3220.12213161388204058No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA3210.12175232315569885No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG3170.12023516025033189No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT3160.11985586952399015No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT3140.11909728807130665No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACCTGTCTCTTATACAC3120.11833870661862318No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCC3060.11606296226057272No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACCTGTCTCTTATACACAT3020.11454579935520576No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATC3020.11454579935520576No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA3010.11416650862886403No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT2950.11189076427081358No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG2940.11151147354447183No Hit
TGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACAT2900.10999431063910486No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACA2870.10885643846007965No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA2850.10809785700739619No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCTGTCTCTTATACAC2840.10771856628105443No Hit
GTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACAC2830.10733927555471269No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTACTGTCTCTTATACACATCTC2810.10658069410202922No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC2800.10620140337568747No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACAT2790.10582211264934573No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTACTGTCTCTTATACACATCTCC2780.10544282192300397No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC2770.10506353119666226No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACACA2760.1046842404703205No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACCTGTCTCTTATACACA2730.10354636829129527No Hit
GGTATCAACGCAGAGTACGGGTGGTATCCTGTCTCTTATACACATCTCCGA2690.1020292053859283No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTCTGTCTCTTATACACATCTCC2660.10089133320690309No Hit
GTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACA2660.10089133320690309No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTCC253.882699E-544.99981313
CGTTCCC253.882699E-544.99981314
TGCCGTC23300.044.32384545
GCCGTCT356.2301697E-638.57126645
TGGACGT301.1375657E-437.49984426
GGACGTA301.1375657E-437.49984427
AGGGTGA504.8547008E-835.999855
ATGCCGT29000.035.53433644
TCTCTGT458.6664477E-734.99985538
CAGGGTG606.3919288E-933.749864
GACAGCC551.12215275E-732.7271410
GGGTGAC551.12215275E-732.727146
TGACAGC551.12215275E-732.727149
GGTGACA551.12215275E-732.727147
GTGACAG551.12215275E-732.727148
CTGTATC352.8145927E-432.1427237
GACGTAT352.8145927E-432.1427228
CCGTGAC352.8145927E-432.1427237
GTGGACG352.8145927E-432.1427225
GAAAGTG352.8145927E-432.1427221
Produced by FastQC (version 0.11.2)