FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_78.35100000041d18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_78.35100000041d18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498637
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC188693.7841154988498644No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT103182.0692407502852777No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG34810.6981030288566632No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC10950.21959862585407822No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA9280.18610732857770282No Hit
GTTCTATGCCATTTGCTTTCAACTCGTTAATGAGATTGTTGTAGTATGTTA8870.17788491427631725No Hit
ATATGGTTCTATGCCATTTGCTTTCAACTCGTTAATGAGATTGTTGTAGTA8830.17708272751520646No Hit
GATTTAAAGTGATCCAAAATTTGACCCTGTCTCCGAATCTTTGGAATAGAA8700.17447562054159638No Hit
GTCATTGATGGACAATTACGAATTCGGAAATGGTTACACCCTCCGGTTTGG8040.1612395389832684No Hit
ATTACGTTTCACAATATGCAACTGACGCACCTCCTCCGACACAACTTAATC6870.13777557622077785No Hit
CTTATAAGGAGTATCAATGTGGACTATGAAAAGGACGGCCAAGTTGTAACA6640.13316300234439082No Hit
ACTTAATGCAATAACGGATGCACGAGTTACTCTTGGATTTTATCGCAATGG6540.13115753544161382No Hit
TGAGCAATCAGCTTTAGTTAAAGGATCACTTGATTTTCTAGGGTTGAACTA6250.1253416814235606No Hit
GTAACATATGGTTCTATGCCATTTGCTTTCAACTCGTTAATGAGATTGTTG6040.121130200927729No Hit
ACATAGAGCACTTAATGGATGGGACTATTTCACGCATAGATATCCAGAAAA6030.12092965423745128No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT5950.11932528071522971No Hit
GTATAGTGGAGGACTACACAAACTACGCTGAGCTTCTATTCCAAAGATTCG5900.11832254726384123No Hit
CTTCCACTTATTTTGCGAGGAACTTAGAGAACCATTTTCCAGAAGCTTTTT5850.11731981381245275No Hit
GAGAAAGGCTGATTTAAAGTGATCCAAAATTTGACCCTGTCTCCGAATCTT5800.11631708036106427No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA5740.11511380021939809No Hit
ATTAAGTTGTGTCGGAGGAGGTGCGTCAGTTGCATATTGTGAAACGTAATC5740.11511380021939809No Hit
GTGTAACCATTTCCGAATTCGTAATTGTCCATCAATGACCATGCAAAATAT5580.11190505317495493No Hit
GGATAACATACTACAACAATCTCATTAACGAGTTGAAAGCAAATGGCATAG5540.11110286641384413No Hit
TCAGCAGATCTTGCTTGTGATTCGTATGATCTTTATAAGGATGATGTCAAA5540.11110286641384413No Hit
ATCTCCTGAACTACGATCCGGTACTTTTTCTGGATATCTATGCGTGAAATA5470.10969903958190025No Hit
GGACTATTTCACGCATAGATATCCAGAAAAAGTACCGGATCGTAGTTCAGG5180.10388318556384706No Hit
CTTAATGGATGGGACTATTTCACGCATAGATATCCAGAAAAAGTACCGGAT5020.1006744385194039No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC20550.043.9037445
GTGTAGA352.8119897E-432.154751
TCTAGCC300.005145280629.99906713
GCTACCG852.0190782E-1029.116747
ATGCCGT31700.028.46125644
GTATTAG1004.5474735E-1127.0099931
TGGACGG755.6164936E-827.0045745
GGACGGA755.6164936E-827.0045746
CGCAATG2650.026.31993144
TATGCCG34350.026.26555643
GTGCATT608.181376E-626.2491849
CGTATGC34350.026.06905741
CTACCGT957.403287E-1026.0518198
GTATGCC34850.025.8887242
ATCTCGT34550.025.8530337
AATCTCG34700.025.8061136
TATTAGC1058.367351E-1125.7186412
TATAACG709.2761184E-725.7186413
ATTACAA35400.025.5500531
TCTCGTA35000.025.52063238
Produced by FastQC (version 0.11.2)