FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_74.35100000041c83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_74.35100000041c83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251365
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC76223.0322439480436816No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC22230.8843713325244166No Hit
ACATACTACAACAATCTCATTAACGAGTTGAAAGCAAATGGCATAGAACCA17540.6977900662383386No Hit
GGATAACATACTACAACAATCTCATTAACGAGTTGAAAGCAAATGGCATAG16270.6472659280329401No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT15710.6249875678793786No Hit
TACTACAACAATCTCATTAACGAGTTGAAAGCAAATGGCATAGAACCATAT13150.5231436357488115No Hit
TAACATAATAGTTCAACCCTAGAAAATCAAGTGATCCTTTAACTAAAGCTG12900.5131979392516858No Hit
GTGTAACATAATAGTTCAACCCTAGAAAATCAAGTGATCCTTTAACTAAAG11470.4563085552881268No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA11000.43761064587353055No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC9530.37912995047043146No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC9090.36162552463549025No Hit
AATACATACAGTGCAATTGTTATTACATTCGAGGTTTGGATTTATATCTAG8520.33894933662204363No Hit
ACAATAATGAGAGAGATGGTAGGAGATAGATTGCCAGAATTCACACCTGAG8340.33178843514411316No Hit
ATATGGTTCTATGCCATTTGCTTTCAACTCGTTAATGAGATTGTTGTAGTA8160.32462753366618263No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC7740.3079187635510115No Hit
TTGTGTAACATAATAGTTCAACCCTAGAAAATCAAGTGATCCTTTAACTAA7530.2995643784934259No Hit
GAATGGGATAACATACTACAACAATCTCATTAACGAGTTGAAAGCAAATGG7350.2924034770154954No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG6890.2741033954607841No Hit
GTACAATTGTTATTACATTCGAGGTTTGGATTTATAAATCTAGGGCTTCAA6870.2733077397410141No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT6660.26495335468342845No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA6000.23869671593101666No Hit
GTAACATATGGTTCTATGCCATTTGCTTTCAACTCGTTAATGAGATTGTTG5660.22517056869492572No Hit
GTTGCATATTGTGTAACATAATAGTTCAACCCTAGAAAATCAAGTGATCCT5570.22159011795596048No Hit
CAACAATAATGAGAGAGATGGTAGGAGATAGATTGCCAGAATTCACACCTG4910.19533347920354863No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC4540.18061384838780262No Hit
CAGTACAATTGTTATTACATTCGAGGTTTGGATTTATAAATCTAGGGCTTC4440.1766355697889523No Hit
ACATAATAGTTCAACCCTAGAAAATCAAGTGATCCTTTAACTAAAGCTGAT4380.17424860262964215No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC4290.17066815189067688No Hit
TGAGCAATCAGCTTTAGTTAAAGGATCACTTGATTTTCTAGGGTTGAACTA4280.17027032403079187No Hit
TAGTAACATATGGTTCTATGCCATTTGCTTTCAACTCGTTAATGAGATTGT4160.16549638971217154No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA3960.15753983251447098No Hit
ACGTAATAGTTCAACCCTAGAAAATCAAGTGATCCTTTAACTAAAGCTGAT3920.15594852107493087No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC3850.1531637260557357No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG3810.15157241461619558No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG3800.15117458675631054No Hit
GAACCATATGTTACTATATTTCATTGGGATGTTCCCCAAACTTTAGAAGAC3710.14759413601734528No Hit
GAAATATAGTAACATATGGTTCTATGCCATTTGCTTTCAACTCGTTAATGA3680.1464006524376902No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA3610.143615857418495No Hit
GTAACATAATAGTTCAACCCTAGAAAATCAAGTGATCCTTTAACTAAAGCT3530.14043323453941478No Hit
ATATAGTAACATATGGTTCTATGCCATTTGCTTTCAACTCGTTAATGAGAT3440.13685278380044955No Hit
AAACGTAATAGTTCAACCCTAGAAAATCAAGTGATCCTTTAACTAAAGCTG3410.13565930022079448No Hit
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC3380.13446581664113938No Hit
GAGTCTGTAAGCTTGAACATTCATTCTTTTTAGCAGTTTGACATCATCCTT3370.13406798878125437No Hit
CTATACGTGTGCTTAAGAAACCTCCATATTCGTCTTCTAAAGTTTGGGGAA3300.13128319376205916No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGT3240.128896226602749No Hit
GCATAGAACCATATGTTACTATATTTCATTGGGATGTTCCCCAAACTTTAG3230.12849839874286398No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT3170.12611143158355378No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACG3090.12292880870447358No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG3060.12173532512481848No Hit
ACATAGAGCACTTAATGGATGGGACTATTTCACGCATAGATATCCAGAAAA2940.11696139080619816No Hit
TCAGCAGATCTTGCTTGTGATTCGTATGATCTTTATAAGGATGATGTCAAA2900.11537007936665805No Hit
CCACTATACGTGTGCTTAAGAAACCTCCATATTCGTCTTCTAAAGTTTGGG2790.11099397290792275No Hit
ACAACAATCTCATTAACGAGTTGAAAGCAAATGGCATAGAACCATATGTTA2740.1090048336084976No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACT2680.10661786644918744No Hit
GTCTGTAAGCTTGAACATTCATTCTTTTTAGTAGTTTGACATCATCCTTAT2550.10144610427068208No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTTT253.882184E-544.99986
TGCCGTC9100.042.03278445
TGGCGTG301.1374157E-437.4998369
ATGCCGT10800.035.4165144
TATGCCG10950.034.9313543
CACGAGA11150.034.91015621
CCCACGA11150.034.91015619
TTACAAT10800.034.7915132
CGTATGC11000.034.7725741
GCCCACG11200.034.75431418
CCACGAG11250.034.59984620
TGATTAC10900.034.47232429
GACTGAT10900.034.47232426
ACGAGAC11300.034.44675422
ATCTCGT11050.034.41161737
GTATGCC11150.034.3047842
CTCGTAT11100.034.25660339
AATCTCG11100.034.053936
GATTACA11050.034.0043830
CAGTAGA401.5557864E-533.74984723
Produced by FastQC (version 0.11.2)