FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_72.35100000041c40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_72.35100000041c40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45003
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC14353.1886763104681908No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT6421.4265715618958736No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG1780.3955291869430927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2050.043.9024445
CCACAAA352.7778148E-432.1428623
ATGCCGT3000.030.044
ACTCAAC3150.029.28571527
GACTCAA3150.029.28571526
GTATCTC3000.029.2535
ACGAGAC3100.029.03225922
CGAGACT3100.029.03225923
ATCTCGT3050.028.77049337
TCTCGTA3050.028.77049338
TATCTCG3050.028.77049336
CACGAGA3150.028.57142821
AGACTCA3150.028.57142825
GAGACTC3150.028.57142824
CTCAACT3250.028.38461728
TGTATCT3100.028.30645434
CTGTATC3100.028.30645433
CTCGTAT3100.028.30645439
CCACGAG3200.028.12520
CCCACGA3200.028.12519
Produced by FastQC (version 0.11.2)