FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_71.35100000041c33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_71.35100000041c33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272004
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC4819917.719960000588227No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT117384.315377715033603No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG87863.23009955735945No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC13660.5021984970809252No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA12650.46506669019573244No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATACA12150.4466846075793003No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACA12150.4466846075793003No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC11200.4117586506080793No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATA10320.3794061852031588No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATA10120.3720533521565859No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG9400.3455831531889237No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG9110.33492154527139306No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA8780.3227893707445479No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC8070.29668681342921427No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCCTGTCTCTTATA7760.28528992220702637No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC7300.2683784061999088No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATA6380.23455537418567374No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA5600.20587932530403966No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC5450.20036470051911No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCCTGTCTCTTATAC5390.19815885060513816No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC5280.1941147924295231No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC5240.19264422582020854No Hit
GTACTCTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTA5100.18749724268760753No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC4990.18345318451199247No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCTGTCTCTTATACAC4560.16764459346186086No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACCTGTCTCTTATACAC4330.1591888354583021No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC4270.15698298554433024No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCC4110.15110071910707196No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG4060.14926251084542874No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCTGTCTCTTATAC3820.14043911118954133No Hit
CTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATA3770.13860090292789812No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA3690.13565976970926896No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACAT3660.13455684475228305No Hit
GTACTCTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTT3630.13345391979529714No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC3410.125365803444067No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA3310.12168938692078057No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGA3260.11985117865913736No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACT3150.1158071204835223No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACT3140.11543947883119365No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAGAGTACCTGTCTCTTA3140.11543947883119365No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA3140.11543947883119365No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTC3130.11507183717886502No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAC3060.11249834561256453No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAGAGTACCTGTCTCTT3030.1113954206555786No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG3000.11029249569859266No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT2950.10845428743694946No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT2900.10661607917530624No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC2860.10514551256599167No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA2830.10404258760900575No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA2820.10367494595667712No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT2810.10330730430434847No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGG2790.10257202099969118No Hit
CTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTAT2780.10220437934736254No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC2760.10146909604270525No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACCTGTCTCTTATACA2730.10036617108571932No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGAAA207.0147344E-445.011771
TGCCGTC48150.044.9017745
TCGATTA403.4381992E-739.38532
GATTACG403.4381992E-739.38534
TACAGGG1050.036.43812
TATTCTG250.002101587436.009425
CCCCTGG250.002105384835.99617814
GAGGGCT250.002105384835.99617841
ATGCCGT60600.035.6397844
GCCTAAC458.6497676E-735.009151
ATACAGG1100.034.7818221
AAAAAGT1850.034.062961
CAGGGTG1150.033.269574
AAAAGTA1900.033.166572
ATTGTGA1100.032.7358324
GGTGACA1250.032.3965577
GTGACAG1250.032.3965578
AGGGTGA1200.031.8833375
GTCGATT501.9702475E-631.508241
CGATTAC501.9702475E-631.508243
Produced by FastQC (version 0.11.2)