FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_70.35100000041c0c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_70.35100000041c0c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364349
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC102482.8126878350153284No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT59171.6239923809314696No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG24260.6658451100455881No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA9350.25662208486917765No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT6560.18004715259270643No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC4600.1262525765131783No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA3800.10429560668479947No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC11150.044.3932645
GTCCTAG250.002102703336.0087851
GTCCTAT300.00513656430.0073221
GGCGTTT406.1590946E-428.12414236
ATGCCGT18050.027.42298744
CCGCCCT507.1466944E-526.99917615
CGCCCTA507.1466944E-526.99917616
CCCGCCC507.1466944E-526.99917614
CCTAGAT608.156014E-626.2564073
ACCCCCG450.001225164624.99923521
CGTGTCC551.3659817E-424.5447049
GTCCCGC551.3659817E-424.54470412
TATGCCG20750.023.85469443
CGTATGC20850.023.74028441
TATCTCG20750.023.52940236
GTATGCC21100.023.45900242
CTCGTAT20950.023.41217439
TCGTATG21050.023.40783740
CCACGAG21300.022.81620620
GTATCTC21350.022.76277435
Produced by FastQC (version 0.11.2)