FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_67.35100000041bba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_67.35100000041bba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154673
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC29651.916947366379394No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT20211.3066275303381973No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT9700.6271294925423313No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT8430.5450207857867889No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC7920.5120479980345632No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA5350.3458910087733476No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA4370.2825315342690709No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC3760.2430934940164088No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT3610.2333956152657542No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC3590.23210256476566693No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG3500.22628383751527414No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC3290.2127068072643577No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC3260.21076723151422677No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT3190.2062415547639213No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT2880.18619927201256847No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT2860.18490622151248118No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG2780.17973401951213205No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGACTGTCTCTTATAC2740.1771479185119575No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA2680.17326876701169563No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT2560.16551046401117195No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC2320.14999385801012458No Hit
TCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATAC2210.14288208025964455No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCT2210.14288208025964455No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAC2160.13964945400942635No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA2100.1357703025091645No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC1910.12348632275833533No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA1870.12090022175816077No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCCCC1870.12090022175816077No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC1770.11443496925772437No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA1760.11378844400768073No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC1680.10861624200733158No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC1670.10796971675728795No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA1670.10796971675728795No Hit
GTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTA1630.10538361575711339No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGCT1570.10150446425685154No Hit
GTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCC1560.10085793900680791No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC207.014592E-444.99967621
TGCCGTC2600.044.134345
TCACGAT250.002101560635.9997420
CGATCAT250.002101560635.9997423
CAGTAAC458.614006E-735.0110661
TGTCCCC352.809295E-432.14262845
CATATTG453.4640565E-530.0094871
TGATCCC300.00513383629.99978425
ATAGGGT300.00513383629.9997846
TACTGAG300.00513383629.9997847
TAACCTA7450.027.4830235
GACACGT755.5735654E-826.99980529
GGGCGGG755.5735654E-826.99980523
GCGGGAC755.5735654E-826.99980525
AAACACC1950.026.5382738
CTGGGTC856.3901098E-926.47039845
ATAGTGC856.3901098E-926.47039827
AACCTAT7900.025.9175366
GAGTAGG801.0415897E-725.31231718
GTAGGGC801.0415897E-725.31231720
Produced by FastQC (version 0.11.2)