FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_63.35100000041b34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_63.35100000041b34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206845
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC3004914.527303053010709No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT82864.005898136285625No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCG58922.848509753680292No Hit
TACCACAAGGCTTGCATTACGTTCTGCAACAAATGCCTGTCTCTTATACAC2920.14116850781986512No Hit
TACCACAAGGCTTGCATTACGTTCCTGTCTCTTATACACATCTCCGAGCCC2910.14068505402596146No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG2870.13875123885034688No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG2740.13246633952959946No Hit
GAACGTAATGCAAGCCTTGTGGTACTGTCTCTTATACACATCTCCGAGCCC2300.11119437259783896No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC2210.10684328845270614No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA2100.101525296719766No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC32450.044.51320345
ATTACGT900.039.9987116
TACGTTC900.039.9987118
AGGCTTG850.039.7046058
GGCTTGC900.037.498799
ACGTTCC552.7266651E-936.81699419
ATAGACC250.002100960636.0075453
TAGGCCT250.002100960636.0075454
GCATTAC1000.035.9988414
GCCGTCT250.002103456535.9988445
GCTTGCA950.035.52517310
ATGCCGT40850.035.30486344
CATTACG1050.034.2846115
TTACGTT1050.034.2846117
ATACTGG401.5521797E-533.7570726
CACAAGG1200.033.7570724
TGCATTA1000.033.74891313
GTGTACG651.3922545E-831.15284219
TATGCCG46750.030.84927243
AAGGCTT1100.030.6808287
Produced by FastQC (version 0.11.2)