FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_62.35100000041b1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_62.35100000041b1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397217
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC378179.520488800831787No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT209565.275705722564744No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCG52971.3335280212075515No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA40591.021859588084095No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT27040.6807362222664186No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA18330.4614606122094472No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGA12540.3156964581072814No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT9360.2356394615537603No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC8740.22003086474143857No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT8450.21273006945825582No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA7680.1933451992235982No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC6510.16389026652937816No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA5520.1389668619419612No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA5420.1364493463270706No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC5110.12864504792090972No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATACAC5050.12713453855197537No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC4990.12562402918304102No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATC4890.12310651356815042No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4850.12209950732219417No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT4640.1168127245309239No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACC4630.11656097296943485No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA4560.11479871203901142No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA4520.11379170579305518No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGC4270.10749791675582866No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC4270.10749791675582866No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT4240.1067426620713615No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA4210.10598740738689433No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC4130.10397339489498184No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACCG351.2095552E-744.998496
AGCTCCG207.028867E-444.9984910
TGCCGTC40650.044.8324445
TGTCCTA250.002104498736.00332333
AGGTCGA250.002104498736.00332329
GGGACGC250.002105799535.9987917
GACATGC250.002105799535.9987911
GGTTATC250.002105799535.998798
GCTCCGA250.002105799535.9987911
GCCTATA352.8176312E-432.14177734
TAACCGT501.9778236E-631.4989417
ATTAGAC300.005144095529.9989933
CTGTACG300.005144095529.9989939
CGACCAA300.005144095529.99899312
TATTCCG300.005144095529.9989935
CGGGACG300.005144095529.99899316
GGACGCA300.005144095529.99899318
CTACCAG300.005144095529.9989936
CAGCTCC453.485952E-529.9989939
GCGTGAT453.485952E-529.99899311
Produced by FastQC (version 0.11.2)