FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_61.35100000041aff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_61.35100000041aff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317985
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC301519.481893800022014No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT97423.0636665251505573No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCG57651.812978599619479No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC19970.6280170448291587No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA14820.4660597197981037No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT11520.36228123968111703No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT10700.33649385977325974No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT9410.2959259084548013No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC8750.275170212431404No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA8650.2720254100036165No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACACT8510.26762268660471406No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCAT7490.23554570184128182No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTGTCTCTTATACA7360.2314574586851581No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT6950.21856376873122946No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATACA6350.1996949541645046No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACA5950.18711574445335472No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC5830.18334198154000975No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC5670.1783102976555498No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT5630.1770523766844348No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGG5430.17076277182885985No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA5330.16761796940107238No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA5300.16667452867273613No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT5240.16478764721606365No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA5200.16352972624494866No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA5150.16195732503105492No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATC5010.15755460163215246No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCTGTCTCTTATA4850.1525229177476925No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT4790.15063603629102001No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT4740.14906363507712628No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATCT4710.14812019434879004No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG4700.1478057141060113No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT4620.1452898721637813No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACCTGTCTCTTATA4510.1418305894932151No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA4490.1412016290076576No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA4450.1399437080365426No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA4340.13648442536597638No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATC4300.1352265043948614No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT4280.1345975439093039No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGA4250.13365410318096765No Hit
GGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATAC4120.12956586002484394No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAAC4110.1292513797820652No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATA4100.12893689953928644No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCG4040.12705001808261396No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCTGTCTCTT4010.12610657735427772No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATACACATCTCC3980.12516313662594147No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC3930.12359073541204774No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT3920.12327625516926899No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCCTGTCTCTTATA3890.12233281444093275No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC3820.12013145274148151No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCCTGTCTCTTATAC3780.11887353177036653No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG3690.1160432095853578No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTT3690.1160432095853578No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC3480.1094391244870041No Hit
GGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACCT3470.10912464424422536No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA3400.10692328254477412No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG3380.10629432205921663No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATT3370.10597984181643788No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT3320.10440744060254414No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC31100.044.77998745
TAGCCTC356.2368817E-638.5688734
GGTGAGT504.8225047E-836.031611
GTGAGTA504.8426955E-836.0146032
ATAACGC250.002100635336.0146033
AACCGCT250.002105507335.99761233
AGTTAAC250.002105507335.99761227
GAACCGC250.002105507335.99761232
AGCCCCG1350.034.99767713
TGACAGC1350.034.9976779
GACAGCC1400.033.7477610
CAGCCCC1400.033.7477612
CCGTACA1400.033.7477617
ATGCCGT41700.033.39706444
CAGGGTG1350.033.346854
AGGGTGA1350.033.3416065
TGAGTAA551.1193515E-732.7405473
ATTCAGC551.1240081E-732.725145
ACAGCCC1450.032.5840411
ACAGGGT1400.032.1558953
Produced by FastQC (version 0.11.2)