FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_60.35100000041ac8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_60.35100000041ac8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348810
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC4518212.953183681660501No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT107013.067859293024856No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCG32020.9179782689716466No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA15190.43548063415613086No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT10470.3001634127461942No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC9560.2740747111608039No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT6250.1791806427568017No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA6160.17660044150110377No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT5400.15481207534187666No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA5050.14477795934749577No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA4940.14162438003497607No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT4730.13560391043834752No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTGTCTCTTATACA4600.13187695306900604No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4340.1244230383303231No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCAT4240.12155614804621427No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA3610.10349473925632866No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGG3560.10206129411427425No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT3490.10005447091539807No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC45850.044.5550845
ATGCCGT57250.035.64367344
TATGCCG60950.033.44299743
GTATGCC61300.033.2887542
CGTATGC61100.033.2872541
AATCTCG60950.033.25843436
CTCGTAT61000.033.2311739
TGATTAA61350.033.2297130
ATCTCGT60950.033.2215237
CTGATTA61300.033.2201129
GACCTGA61300.033.2201126
TTAAATC61400.033.16600433
CCTGATT61400.033.16600428
ATTAAAT61550.033.15829532
TCGTATG61150.033.1496640
CACGAGA61650.033.14100621
CGAGACC61900.033.0435123
ACGAGAC61900.033.0435122
GATTAAA61900.033.0435131
GAGACCT61700.033.00474524