FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_59.35100000041abb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_59.35100000041abb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232772
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC61012.6210197102744317No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT52452.2532778856563502No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT17010.7307579949478459No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA11450.49189765092021376No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT9370.40253982437750246No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCG8890.3819187874830306No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC6670.2865464918460983No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC4760.20449194920351246No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC3810.16367948034987026No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA3390.14563607306720738No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT3340.1434880483906999No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA3270.14048081384358943No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT3240.13919199903768495No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT3180.13661436942587596No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC3160.13575515955527298No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA3060.131459110202258No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA2840.12200780162562508No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT2800.12028938188441908No Hit
AACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATAC2580.11083807330778617No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC2490.1069716288900727No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACTGCTGTCTCTTATA2460.1056828140841682No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC2420.10396439434296222No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT2410.10353478940766071No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGCTGTCTCTT2390.10267557953705772No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATACAC2370.10181636966645473No Hit
GTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCC2370.10181636966645473No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAC2370.10181636966645473No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCAG207.01673E-445.00644727
TGCCGTC6250.043.91685545
TATATAG250.002102185736.0051572
GCATTGC250.002102185736.00515730
GGGACTG250.002104404435.99742544
TGTCCCC401.5560086E-533.7475845
GCGGTAT352.8146987E-432.1405538
TATCGCG801.0004442E-1030.93528240
GTCACCC1100.030.68621431
TCACGCA300.00513532630.0042974
TGATATA300.00513532630.0042972
TATGCGG300.00513532630.0042975
TATTAGG300.00513532630.0042972
GTCCCTT453.480719E-529.99785445
ATCGCGC852.0190782E-1029.1155641
GTATCAC406.146106E-428.1290281
GGGGCAG406.153838E-428.12298418
GACCGTT406.153838E-428.1229847
TCGCGCG803.3687684E-928.12298442
TAACCTA10050.027.765175
Produced by FastQC (version 0.11.2)