FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_57.35100000041a78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_57.35100000041a78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277356
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC30311.0928193368811203No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT11300.4074186244393487No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC10540.3800170178398881No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA4980.17955263271751828No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC4110.148185004110241No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA3890.14025296009460766No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC3830.1380896753630713No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG3730.13448420081051068No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT3220.11609628059245157No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA3140.11321190095040308No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC3090.11140916367412279No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT2960.10672204675579401No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4200.039.6353945
ATGCCGT4950.033.63002844
CGTATGC5250.031.70831541
TATGCCG5250.031.70831543
GTATGCC5250.031.70831542
TCGTATG5250.031.70831540
CTCGTAT5350.031.11563539
CCCACGA5400.030.82752819
CACGAGA5450.030.54470421
CCTGATT5400.030.4219128
CGAGACC5500.030.26702723
GACCTGA5450.030.13737326
CTTTAGG453.4602097E-530.026831
CCGATTC300.005122695630.0214143
TCGAGGC300.00513627330.00516933
ATCTCGT5550.029.99435237
GCCCACG5600.029.72654518
GAGACCT5600.029.72654524
TCTCGTA5600.029.72654538
AATCTCG5700.029.20502736
Produced by FastQC (version 0.11.2)