FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_56.35100000041a5f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_56.35100000041a5f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131521
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC24191.8392500057025112No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC7620.5793751568190632No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT5620.4273081865253458No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA4430.3368283392005839No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA3630.276001551083097No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC3500.26611719801400535No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC2330.17715802039218073No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC2220.16879433702602628No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCG2090.15890998395693465No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT2030.15434797484812313No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC2010.15282730514518594No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG1840.13990161267021997No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC1790.13609993841287704No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC1670.12697592019525397No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA1450.11024855346294508No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA1440.1094882186114765No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG1390.10568654435413355No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACT207.010788E-444.9996232
GAGCTCG207.010788E-444.999629
CCTAAGT207.010788E-444.9996234
TACGCAG207.010788E-444.9996233
ACTACGC207.010788E-444.9996220
GATTGTA253.872119E-544.9996223
GATGAGA207.010788E-444.9996211
TACTACG207.010788E-444.9996219
TGCCGTC2850.042.63121845
GGATCCC301.13448936E-437.4996839
GTCTATT301.13448936E-437.49968326
TAGCATA301.13448936E-437.4996834
ATGCCGT3250.037.384344
GCCCCTA250.002100431435.99969511
GTTTCCG250.002100431435.99969544
ACGCATA250.002100431435.99969523
TCACAGG250.002100431435.99969526
TAGGTAC250.002100431435.9996958
CTAAGTA250.002100431435.99969535
TGTTGAC250.002100431435.99969531
Produced by FastQC (version 0.11.2)