FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_55.35100000041a35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_55.35100000041a35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128955
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC2644720.50870458687139No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT70615.47555348765073No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCG55954.338722810282657No Hit
ATCAACTCCTGAGTCTCCTTTATCTTCACCATTTACAGGCTTCCCCTGTTT2360.1830095769842193No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC2260.17525493389166763No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGCTGTCTCTT1840.14268543290295066No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA1800.13958357566592997No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT1750.13570625411965415No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCTGTCTCTT1740.13493078981039897No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT1700.1318289325733783No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT1400.10856500329572331No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA1360.10546314605870265No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC1360.10546314605870265No Hit
CTTTCACATTGTTGGCGATAGCTAGTGGTCCATAGCAGTTCAGTTCGTTGT1330.10313675313093715No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG1300.10081036020317166No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC25450.044.82047745
GGTGAGT301.1295388E-437.526841
AGGGTGA250.002096817436.011795
CAGGGTG250.002096817436.011794
ACAGGGT250.002096817436.011793
GTCGCAA250.002100815335.9978318
GCTGTCA250.002100815335.9978328
TGTACGG250.002100815335.9978320
ATGCCGT34250.033.2388744
GAGTAAT352.8012082E-432.1533854
GTAATGT352.8012082E-432.1533856
TGTCACC352.8075956E-432.1409230
TGGAGGG352.8075956E-432.1409229
GTCTGGG352.8075956E-432.1409211
TCTGGGA352.8075956E-432.1409212
GGAGGGG352.8075956E-432.1409230
ATGGGTT300.005132038629.99819211
CTGTCAC300.005132038629.99819229
TGCCTGA300.005132038629.99819222
CACCCTG300.005132038629.99819233
Produced by FastQC (version 0.11.2)