FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_54.35100000041a1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_54.35100000041a1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143348
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC14000.9766442503557775No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT7540.525992689120183No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT7040.4911125373217624No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA6380.4450707369478472No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT3680.2567179172363758No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC3480.24276585651700758No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC1800.12556854647431426No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT1630.11370929486285125No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA1620.11301169182688284No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC1580.1102212796830092No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT1530.10673326450316711No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1550.040.6413845
CAGTAGT301.1355645E-437.49651318
TCACACT250.00210193235.996654
GGCGAAC1050.032.15108529
CCGGGGG1000.031.50806435
CGAACCG1100.030.6896731
GCGAACC1100.030.6896730
GAACCGG1050.030.00767932
GGGGAAC1050.029.9972138
GTACAGG602.3960092E-729.9972113
GGAAGGG300.00513474729.997213
TATACTT453.4719895E-529.9972085
AGGCGAA1150.029.3553428
CGGGGGA1100.028.63369836
TGAGGCG1200.028.13219826
AAGCCTC406.1429694E-428.1223835
ACCAAGT803.3451215E-928.1223838
AGGGAGT406.1429694E-428.1223836
ATGCCGT2250.027.99739344
CACCAAG654.8273614E-727.689737
Produced by FastQC (version 0.11.2)