FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_53.351000000419f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_53.351000000419f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149914
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC3027520.19491174940299No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT1765911.779420200915192No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCG21481.4328214843176754No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA4210.28082767453339913No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC3570.23813653161145723No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT2970.1981135851221367No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA2330.15542244220019477No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA2230.14875195111864137No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1950.13007457609029174No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA1820.12140293768427232No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA1660.11073015195378684No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1570.10472670998038876No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA1560.10405966087223342No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1520.10139146443961206No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC33550.044.45887445
GCCGTCT401.5526683E-533.746545
AAAGAGT300.005119022430.0169096
GAGAGTG300.005135763429.9968877
ATGCCGT51400.029.01936144
ATCTCGT53550.027.85425437
TATCTCG53550.027.8122436
TCTCGTA53550.027.8122438
CGTGTAT53550.027.8122432
GTATCTC53400.027.806135
CTCGTAT53650.027.80233239
CGATCGT53850.027.7826328
TCGTGTA53650.027.76039931
ACGATCG53900.027.75685927
GATCGTG53750.027.7506129
TGTATCT53750.027.7506134
TATGCCG53900.027.7151243
GTATGCC53900.027.7151242
ATCGTGT53750.027.70875430
GTGTATC53750.027.70875433