Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_1228_pa_53.351000000419f0.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 149914 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC | 30275 | 20.19491174940299 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT | 17659 | 11.779420200915192 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCG | 2148 | 1.4328214843176754 | No Hit |
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA | 421 | 0.28082767453339913 | No Hit |
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC | 357 | 0.23813653161145723 | No Hit |
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT | 297 | 0.1981135851221367 | No Hit |
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA | 233 | 0.15542244220019477 | No Hit |
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA | 223 | 0.14875195111864137 | No Hit |
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA | 195 | 0.13007457609029174 | No Hit |
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA | 182 | 0.12140293768427232 | No Hit |
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA | 166 | 0.11073015195378684 | No Hit |
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA | 157 | 0.10472670998038876 | No Hit |
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA | 156 | 0.10405966087223342 | No Hit |
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA | 152 | 0.10139146443961206 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 3355 | 0.0 | 44.458874 | 45 |
GCCGTCT | 40 | 1.5526683E-5 | 33.7465 | 45 |
AAAGAGT | 30 | 0.0051190224 | 30.016909 | 6 |
GAGAGTG | 30 | 0.0051357634 | 29.996887 | 7 |
ATGCCGT | 5140 | 0.0 | 29.019361 | 44 |
ATCTCGT | 5355 | 0.0 | 27.854254 | 37 |
TATCTCG | 5355 | 0.0 | 27.81224 | 36 |
TCTCGTA | 5355 | 0.0 | 27.81224 | 38 |
CGTGTAT | 5355 | 0.0 | 27.81224 | 32 |
GTATCTC | 5340 | 0.0 | 27.8061 | 35 |
CTCGTAT | 5365 | 0.0 | 27.802332 | 39 |
CGATCGT | 5385 | 0.0 | 27.78263 | 28 |
TCGTGTA | 5365 | 0.0 | 27.760399 | 31 |
ACGATCG | 5390 | 0.0 | 27.756859 | 27 |
GATCGTG | 5375 | 0.0 | 27.75061 | 29 |
TGTATCT | 5375 | 0.0 | 27.75061 | 34 |
TATGCCG | 5390 | 0.0 | 27.71512 | 43 |
GTATGCC | 5390 | 0.0 | 27.71512 | 42 |
ATCGTGT | 5375 | 0.0 | 27.708754 | 30 |
GTGTATC | 5375 | 0.0 | 27.708754 | 33 |