FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_50.35100000041993.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_50.35100000041993.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277777
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC67942.4458468483711755No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT16590.5972416722766825No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT9880.35568099590678853No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT5360.19296054028951282No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC5020.18072050601741685No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC4970.17892050097740275No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC4850.17460048888136886No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT4300.15480043344121364No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCG4150.1494004183211713No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA4000.14400040320112897No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT3910.14076039412910357No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT3840.1382403870730838No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT3810.13716038404907532No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC3410.12276034372896245No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT3280.11808033062492575No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC3260.1173603286089201No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT3040.10944030643285801No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA3030.10908030542485518No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCT2780.10008028022478463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7400.044.69219245
ATGCCGT9000.036.7469144
CGGGCAT250.002103372536.0034564
CGTATGC9400.035.18321641
TATGCCG9450.034.99706343
TCGTGTA9350.034.89639731
CGATCGT9400.034.71077728
TATCTCG9400.034.70453336
GATCGTG9450.034.52712629
ACGATCG9450.034.52712627
CGAGACG9600.034.45643223
CTCGTAT9600.034.45023339
TCGTATG9600.034.45023340
ATCGTGT9500.034.34540630
GAGACGA9650.034.27790524
AGACGAT9650.034.0447225
CACGAGA9800.033.98285321
ATCTCGT9600.033.9815237
CCACGAG9800.033.97673820
GACGATC9700.033.86923226
Produced by FastQC (version 0.11.2)