FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_48.35100000041950.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_48.35100000041950.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154662
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC12710.8217920368286974No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG4800.31035419172130196No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA4060.2625079204976012No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT3310.2140150780411478No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT2350.1519442396968874No Hit
CTCATGGACTATGCCTTTGAGTACATTGTTAAGAACGGAGGTCTACGCAAG2010.12996081778329518No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA1960.12672796161953162No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTGTCTCTTATACA1660.10733082463695025No Hit
TCCTTACTCTATGGAAGAAGGAACTTGCGAGATGCAAAAGGATGAATCTGA1600.10345139724043396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT2800.043.39254816
CGTCTTC2900.041.8962518
GTCTTCT3100.040.64486719
CCGTCTT3000.040.4997117
CTGCTTG3150.039.99971424
CTTCTGC3300.037.4997321
TCTGCTT3300.037.4997323
TGAAAAA3250.037.38434629
GCTTGAA3250.037.38434626
TCTTCTG3450.036.5214820
GCATACA250.00209822636.0113871
GCCTCGT250.002101560635.9997413
TGCTTGA3400.035.7350425
TTGAAAA3550.034.2251128
GTCTTAC401.548525E-533.7606741
GTCGGTA401.5519596E-533.74975620
TTCTGCT3700.033.4457122
CTTGAAA3650.033.2874327
CCCATAA352.8039658E-432.1530231
TTAGAGG352.809295E-432.14262821
Produced by FastQC (version 0.11.2)