FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_47.35100000041929.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_47.35100000041929.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149684
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC71184.755351273349189No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG54343.6303145292750063No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT17301.1557681515726463No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAA7790.5204297052457176Illumina PCR Primer Index 8 (96% over 27bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA3180.21244755618502978No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACAA3010.20109029689211938Illumina PCR Primer Index 8 (96% over 27bp)
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT2560.17102696346970953No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC2400.16033777825285267No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA2310.1543251115683707No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCTGTCTCTT2210.14764437080783516No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG2170.14497207450362096No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT2130.14229977819940676No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAAAAAA2040.13628711151492476Illumina PCR Primer Index 8 (96% over 27bp)
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC2000.13361481521071056No Hit
GATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTC1920.12827022260228216No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG1910.1276021485262286No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA1890.1262660003741215No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA1780.11891718553753242No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTGTCTCTTATACA1760.11758103738542529No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTAT1630.10889607439672912No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA1600.10689185216856845No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGAAAA1530.10221533363619358Illumina PCR Primer Index 8 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT17250.044.8655717
GCCGTCT17200.044.8651916
CGTCTTC17350.044.60697618
CTGCTTG16700.044.59183524
GCTTGAA16650.044.59062226
CTTGAAA16900.044.46349327
TGCTTGA16700.044.45711525
GTCTTCT17650.043.84878519
CTTCTGC17550.043.84224721
TCTGCTT17250.043.82218623
TTGAAAA17200.043.68796528
GAAAAAA17850.042.97936630
TTCTGCT17550.042.94489322
TGAAAAA17900.042.8593129
TCTTCTG18000.042.621220
TATAAAC301.13131566E-437.5217322
TGCCAGT250.00210218935.9967932
AGTGCAT250.00210218935.996798
AGCGTCG401.5524955E-533.74699417
TCGGTAA401.5524955E-533.74699421
Produced by FastQC (version 0.11.2)