FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_45.351000000418f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_45.351000000418f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212741
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC65243.066639716838785No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG32231.5149877080581553No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT23361.0980488011243719No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAA12070.5673565509234233Illumina PCR Primer Index 8 (96% over 27bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACAA4030.18943222039945284Illumina PCR Primer Index 8 (96% over 27bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGAAAA2970.1396063758278846Illumina PCR Primer Index 8 (96% over 27bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAAAAAA2650.12456461142892061Illumina PCR Primer Index 8 (96% over 27bp)
TACCACAAGGCTTGCATTACGTTCCTGTCTCTTATACACATCTCCGAGCCC2240.10529235079274799No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACA2130.10012174428060412Illumina PCR Primer Index 8 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATG207.0232037E-444.996244
GCCGTCT23150.044.51032316
CCGTCTT23300.044.2237717
TGAAAAA23350.044.1394529
CTTCTGC23300.043.94442721
CGTCTTC23600.043.56627718
CTGCTTG23100.043.44814324
GTCTTCT23600.043.37561419
TGCTTGA23150.043.354325
CTTGAAA23100.043.35072327
GAAAAAA23800.043.3048830
GCTTGAA23400.042.89111326
TTCTGCT23250.042.78067822
TCTTCTG24250.042.3057420
TTGAAAA23900.041.9938128
TCTGCTT24400.041.59441823
GCCGTCC403.442783E-739.37170816
TAAGGCC250.002104121535.9969912
CCGTCCT458.6613363E-734.99707417
TGCCGTC31250.033.47720315
Produced by FastQC (version 0.11.2)