FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_43.351000000418bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_43.351000000418bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222344
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC43161.9411362573309825No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA29521.327672435505343No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT25481.1459720073399777No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG22971.0330838700392186No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT18600.8365415752167812No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC13330.5995214622386933No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA7490.3368653977620264No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT7190.3233727917101428No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA6420.28874176951030833No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC6060.2725506422480481No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATACAC5490.24691469074946928No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGA5390.24241715539884143No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC5010.22532652106645557No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT4560.20508761198863024No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC4430.19924081603281402No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT4260.19159500593674667No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG4200.18889648472636994No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA3590.16146151908754003No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC3450.15516496959666104No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA3400.1529162019213471No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC3340.15021768071097039No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT3250.14616989889540533No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCTCTTATAC3200.1439211312200914No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC3160.14212211707984024No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA3050.1371748281941496No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTCTGTCTCTTATACACATCT3040.13672507465908681No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2930.1317777857733962No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC2930.1317777857733962No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC2790.12548123628251717No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT2720.12233296153707769No Hit
AACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATAC2690.12098370093188932No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGCT2610.11738567265138704No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA2540.11423739790594753No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA2530.11378764437088476No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA2500.1124383837656964No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT2490.1119886302306336No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG2420.10884035548519412No Hit
AACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCC2340.10524232720469182No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC2260.10164429892418954No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC2260.10164429892418954No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCA207.024285E-444.99572823
CCGTCTT4600.044.99572817
CGCAGGG207.024285E-444.99572826
ATGACGC207.024285E-444.99572822
GCCGTCT4650.044.0280816
CGTCTTC4800.042.65220318
GTCTTCT4850.042.21248619
GCTTGAA5050.040.09520326
CTTCTGC5150.039.75350621
TGCTTGA5100.039.7021125
TTCTGCT5000.039.14628222
TCTGCTT5150.038.87980323
GTCCAAA356.2296385E-638.56776411
TTGAAAA5300.038.20392228
CTGCTTG5350.037.84687424
TCTTCTG5400.037.4964420
CTTGAAA5500.037.22373627
TGAAAAA5800.036.4620529
CATTAGA250.002095163636.0289961
CCGTCCT250.002104443335.9965817
Produced by FastQC (version 0.11.2)