FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_42.35100000041894.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_42.35100000041894.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309622
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC42401.3694117343082857No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT22530.7276614710840961No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG13590.4389222988030566No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC10890.351719193080595No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA5890.19023196026122174No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA5310.17149944125417446No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC4390.14178579041540976No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC4310.13920199469029979No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT4220.13629522449955106No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG3430.11078024171409008No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA207.0282765E-444.996129
TGCTGGC207.0282765E-444.9961217
GCCGTCT6450.044.29850816
CCGTCTT6750.041.99638417
CTGCTTG6700.040.6373924
CGTCTTC7300.038.8322718
TGCTTGA7050.038.6199325
TGAAAAA7400.037.71163629
TATGCGG301.1385733E-437.4967735
GCTTGAA7450.036.55228426
GTCTTCT8000.035.7156719
TCTGCTT7750.035.71236823
CTTCTGC8350.034.49361821
GAAAAAA8250.034.37177730
TTCTGCT8150.034.2357122
TTGAAAA8050.033.5483228
CTTGAAA8150.033.13668427
GCGGTAT352.8170735E-432.1400878
TGCGGTA352.8170735E-432.1400877
TCTTCTG9500.030.07635920
Produced by FastQC (version 0.11.2)