FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_41.3510000004186d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_41.3510000004186d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213358
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC53562.510334742545393No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA23331.0934673178413745No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT17260.8089689629636573No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG16390.7681924277505413No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT12620.5914941084937054No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAA8630.40448448148182864Illumina PCR Primer Index 8 (96% over 27bp)
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA7980.3740192540237535No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGA5100.2390348615941282No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATACAC4250.19919571799510682No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC4040.1893531060471133No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT3880.18185397313435636No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA3460.16216874923836933No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTGTCTCTTATACA3390.15888787858903813No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA3150.1476391792199027No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACAA3020.14154613372828764Illumina PCR Primer Index 8 (96% over 27bp)
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT2970.1392026546930511No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTCTGTCTCTTATACACATCT2870.134515696622578No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCTGTCTCTTATACAC2770.12982873855210492No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCTCTTATAC2760.1293600427450576No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTCTGTCTCTTATACACATCTC2710.12701656370982106No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA2660.1246730846745845No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC2650.1242043888675372No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2490.11670525595478022No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC2440.11436177691954368No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT2350.11014351465611788No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTCTGTCTCT2230.10451916497155017No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAT207.020361E-445.0000041
CCGTCTT16550.044.45619217
GCCGTCT16500.044.45454416
CGTCTTC16650.044.1891918
GCTTGAA16450.044.04255326
CTGCTTG16400.043.9024424
CTTCTGC16700.043.65269521
GTCTTCT16700.043.65269519
TGCTTGA16700.043.11377325
TTCTGCT16650.043.1081122
CTTGAAA16950.042.87610627
TTGAAAA16950.042.87610628
TCTGCTT16950.042.87610623
TCTTCTG17100.042.49999620
GAAAAAA18600.039.91935730
TGAAAAA18600.039.6774229
CGGGTGT250.002103272636.016
AAACTCG250.002103272636.06
CGTTATG250.002103272636.02
TTATAAT250.002103272636.01
Produced by FastQC (version 0.11.2)