FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_39.35100000041837.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_39.35100000041837.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123819
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC5750746.44440675502145No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT1758514.202182217591808No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCG90057.272712588536493No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA1380.11145300801977079No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGC1350.10903011654108012No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC1320.10660722506238947No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCATT207.010445E-444.9979829
GTCACCC207.010445E-444.99798231
TATCGCG207.010445E-444.99798240
TCGCGCG207.010445E-444.99798242
GTATCGC207.010445E-444.99798239
TCACCCT207.010445E-444.99798232
ATCGCGC207.010445E-444.99798241
TGTATCG207.010445E-444.99798238
CGCGCGC207.010445E-444.99798243
CGCGCCT207.010445E-444.99798245
TGCCGTC60200.044.73636645
GCGCGCC250.00210033235.99838644
TGTCACC250.00210033235.99838630
CCCTGTA250.00210033235.99838635
CTGTCAC250.00210033235.99838629
GCATTTT250.00210033235.99838611
ATTTTTG250.00210033235.99838613
AGCCTTG250.00210033235.99838635
ATGCCGT78150.034.40344644
TTTTGTG352.8066963E-432.14141515
Produced by FastQC (version 0.11.2)