FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_38.3510000004181d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_38.3510000004181d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243492
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC6569826.981584610582686No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT3469214.24769602286728No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCG108584.4592840832553025No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA7720.3170535376932302No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT5810.23861153549192582No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA4510.18522169106171865No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT3160.12977839107650355No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC3010.12361802441147965No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTGCG207.022323E-445.07
TGCCGTC67300.044.76597645
GCCGTCT403.4432742E-739.37545
GACAGCC551.1215161E-732.72727610
GAGCTCG551.1215161E-732.7272769
TGAGCTC551.1215161E-732.7272768
GGGACAC501.9731488E-631.527
GGGCGGG501.9731488E-631.523
GCGGGAC501.9731488E-631.525
GGCGGGA501.9731488E-631.524
AGCTCGA501.9731488E-631.510
GGACACG501.9731488E-631.528
AGGGCGG501.9731488E-631.522
CGCTGAA300.005139385430.00000227
TATGAGA300.005139385430.0000022
GCACATA602.4057408E-730.00000231
TGTTATC300.005139385430.0000022
ATGCCGT104150.028.94863344
GTAGGGC554.1472376E-628.63636620
ATGAGTA554.1472376E-628.63636616