FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_37.351000000417e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_37.351000000417e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC372.5342465753424657No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA241.643835616438356No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT161.095890410958904No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC161.095890410958904No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC140.9589041095890412No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC140.9589041095890412No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA130.8904109589041096No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG120.821917808219178No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC110.7534246575342466No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC110.7534246575342466No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA100.684931506849315No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC90.6164383561643836No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA80.547945205479452No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG80.547945205479452No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT70.4794520547945206No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC70.4794520547945206No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG70.4794520547945206No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC60.410958904109589No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC60.410958904109589No Hit
CTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGA60.410958904109589No Hit
GTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGA60.410958904109589No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACT50.3424657534246575No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTT50.3424657534246575No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT50.3424657534246575No Hit
CAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGG50.3424657534246575No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACG50.3424657534246575No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTC40.273972602739726No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTA40.273972602739726No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCAT40.273972602739726No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAG40.273972602739726No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTG40.273972602739726No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA40.273972602739726No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGT40.273972602739726No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT40.273972602739726No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCA40.273972602739726No Hit
ACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGCTTC40.273972602739726No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCA30.2054794520547945No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCAC30.2054794520547945No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTC30.2054794520547945No Hit
GGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGAG30.2054794520547945No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGC30.2054794520547945No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT30.2054794520547945No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA30.2054794520547945No Hit
CAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC30.2054794520547945No Hit
GTACGGGGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTT30.2054794520547945No Hit
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC30.2054794520547945No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGG30.2054794520547945No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG30.2054794520547945No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCAT30.2054794520547945No Hit
GTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA30.2054794520547945No Hit
GGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGA30.2054794520547945No Hit
GTACGGGATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAAC30.2054794520547945No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGC30.2054794520547945No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT30.2054794520547945No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTAC30.2054794520547945No Hit
CTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCC30.2054794520547945No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC30.2054794520547945No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG30.2054794520547945No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATC30.2054794520547945No Hit
CCACTGCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG30.2054794520547945No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTT20.136986301369863No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA20.136986301369863No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT20.136986301369863No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCA20.136986301369863No Hit
GTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGG20.136986301369863No Hit
TTATCGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCTTGC20.136986301369863No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT20.136986301369863No Hit
GTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATT20.136986301369863No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACA20.136986301369863No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC20.136986301369863No Hit
CTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTC20.136986301369863No Hit
ATTCTGATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAG20.136986301369863No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACCTG20.136986301369863No Hit
ATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATA20.136986301369863No Hit
CCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC20.136986301369863No Hit
GTACGGGGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTA20.136986301369863No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA20.136986301369863No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCC20.136986301369863No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTCTAATCTCGTATGCCGTC20.136986301369863No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATG20.136986301369863No Hit
AAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTA20.136986301369863No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCG20.136986301369863No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTG20.136986301369863No Hit
ATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCC20.136986301369863No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA20.136986301369863No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGT20.136986301369863No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT20.136986301369863No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT20.136986301369863No Hit
AGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGG20.136986301369863No Hit
AACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTC20.136986301369863No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATA20.136986301369863No Hit
TAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC20.136986301369863No Hit
GCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATAC20.136986301369863No Hit
CAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC20.136986301369863No Hit
CTCATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGA20.136986301369863No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATG20.136986301369863No Hit
CGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATGCA20.136986301369863No Hit
CTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTA20.136986301369863No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTC20.136986301369863No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCT20.136986301369863No Hit
CTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCCCG20.136986301369863No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA20.136986301369863No Hit
CTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG20.136986301369863No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC20.136986301369863No Hit
GACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCAT20.136986301369863No Hit
CTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATACACG20.136986301369863No Hit
GTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTAC20.136986301369863No Hit
GTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTAC20.136986301369863No Hit
GGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACCTG20.136986301369863No Hit
GTATGCGCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGA20.136986301369863No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGC20.136986301369863No Hit
GTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTAC20.136986301369863No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT20.136986301369863No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAA20.136986301369863No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCAC20.136986301369863No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC20.136986301369863No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTC20.136986301369863No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACATACTGATT20.136986301369863No Hit
GCGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATT20.136986301369863No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAA20.136986301369863No Hit
TTTCCGAATGGGGAAACCCAGTGTGTTTCGACACACTATCATTAACTGAAT20.136986301369863No Hit
GTACGGGACGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAG20.136986301369863No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTA20.136986301369863No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCCTGTCTCTTATA20.136986301369863No Hit
TGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTC20.136986301369863No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCT20.136986301369863No Hit
ATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCA20.136986301369863No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT20.136986301369863No Hit
AGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAG20.136986301369863No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)