FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_34.35100000041788.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_34.35100000041788.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92280
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC22942.4859124403987862No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC5220.5656697009102731No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT3350.36302557433896837No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC3110.33701777199826616No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCA3090.33485045513654094No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCC2980.32293021239705244No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGA2700.2925877763328999No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT2530.2741655830082358No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGCCC2240.24273948851322064No Hit
GTATCAACGCAGAGTACGGGCATCCTCCAAGGCTAAATACTCCTGACTGAC2200.23840485478977025No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTC1970.21348071087993067No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGCTGTCTC1970.21348071087993067No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCA1860.20156046814044215No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTC1820.19722583441699176No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAG1760.1907238838318162No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCG1630.17663632423060252No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGG1600.17338534893801474No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA1470.15929778933680105No Hit
TATCAACGCAGAGTACGGGCATCCTCCAAGGCTAAATACTCCTGACTGACC1450.15713047247507583No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA1420.15387949718248808No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTC1400.15171218032076292No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC1400.15171218032076292No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG1380.14954486345903772No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGG1290.1397919375812744No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTG1290.1397919375812744No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATG1280.13870827915041178No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTT1280.13870827915041178No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT1230.1332899869960988No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCG1170.1267880364109233No Hit
TCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCACCT1170.1267880364109233No Hit
GGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAA1170.1267880364109233No Hit
TTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCC1150.1246207195491981No Hit
TTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTA1130.1224534026874729No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCAT1090.11811876896402254No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1080.11703511053315993No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTACC1070.11595145210229736No Hit
GCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTG1060.11486779367143476No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAA1050.11378413524057217No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTC1040.11270047680970957No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGCTGTCTCTTATAC1010.10944950151712181No Hit
GGTATCAACGCAGAGTACGGGCATCCTCCAAGGCTAAATACTCCTGACTGA990.10728218465539661No Hit
GTATCAACGCAGAGTACGGGATCCTCCAAGGCTAAATACTCCTGACTGACC990.10728218465539661No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCAC990.10728218465539661No Hit
AAACCGACACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGAACTC980.10619852622453403No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGCTGTCTCTT970.10511486779367143No Hit
TTTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGT960.10403120936280884No Hit
GTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA960.10403120936280884No Hit
GATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGCATTTG950.10294755093194624No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTGCA207.007874E-444.9891622
TGCCGTC1850.042.5803945
GTGCTGC250.00209399636.0108451
GCGGGTA250.00209399636.01084522
CGGGGGC250.002099580835.99132516
TTCTGCG250.002099580835.99132518
TCTGCGG250.002099580835.99132519
CTGCGGG250.002099580835.99132520
CTGCATG250.002099580835.9913254
GTGCTTC250.002099580835.99132511
CAGTATG458.5648935E-735.0105441
ATGCCGT2250.035.01054444
CTACTTA2450.033.07118631
ATCTCGT2400.032.80459237
TATCTCG2400.032.80459236
GTATGCC2450.032.15254242
TCTACTT2450.032.15254230
CTCTACT2450.032.15254229
AGGGAGA352.8044067E-432.13511312
CGTATGC2450.032.13511341
Produced by FastQC (version 0.11.2)