FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_33.3510000004176e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_33.3510000004176e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315799
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC86442.7371840949464694No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT42841.3565590771345064No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT20200.6396473706376524No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT9390.2973410302122553No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT8460.2678919185937891No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC7910.250475777314051No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCG7760.24572592060139517No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC7500.23749283563279175No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT7440.2355928929477294No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT7090.22450989395153245No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACTGTCTCTTAT6070.1922108683054728No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGACTGTCTCTTATAC5560.17606135548244295No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA5480.17352809856902648No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC5370.17004487031307888No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA5340.16909489897054772No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT5000.15832855708852783No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4370.13837915889537333No Hit
ACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACA3700.1171631322455106No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA3500.11082998996196948No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC3440.10893004727690714No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG3370.10671344747766776No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT3290.10418019056425132No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAA207.029894E-444.99429715
TGCCGTC10000.043.65138245
GTCTAAT301.13148315E-437.5368581
TAACCTA13000.032.3715365
TAGTGCT1400.032.14387528
ATGCCGT13950.031.29131544
AACCTAT13500.031.172596
TGATAGC1450.031.03546725
ACCTATG13800.030.648297
GATCTAC300.005117026630.0294841
TCTAATA453.4783938E-530.0057032
GCGGACC300.005140875530.0009545
CTGGGTC300.005140875530.0009545
GGATCTT300.005144858729.996240
TATGCCG14600.029.89820943
CCTATGG14100.029.8366458
ATTAACC14500.029.7987673
CGTATGC14700.029.69011541
TCAGTTA14200.029.62652817
ATCTCGT14750.029.58947237
Produced by FastQC (version 0.11.2)