Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_1228_pa_30.3510000004171e.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 405806 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC | 20538 | 5.061039018644377 | RNA PCR Primer, Index 13 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT | 7996 | 1.9703996490934093 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG | 3321 | 0.8183713399999013 | No Hit |
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA | 813 | 0.2003420353567961 | No Hit |
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT | 551 | 0.13577916541401555 | No Hit |
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC | 421 | 0.10374415361034584 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 2075 | 0.0 | 44.348793 | 45 |
GGGGCGA | 30 | 1.1387127E-4 | 37.499485 | 8 |
TTGTCGC | 25 | 0.0021056472 | 35.999508 | 19 |
CCGGGAT | 35 | 2.817409E-4 | 32.142418 | 11 |
GGGCGAA | 35 | 2.817409E-4 | 32.142418 | 9 |
CACCGGG | 35 | 2.817409E-4 | 32.142418 | 9 |
ATGCCGT | 2885 | 0.0 | 31.897308 | 44 |
TACCCAG | 30 | 0.005143726 | 29.99959 | 5 |
ACCGCGA | 45 | 3.4856646E-5 | 29.999588 | 8 |
TACCGCG | 45 | 3.4856646E-5 | 29.999588 | 7 |
TATGCCG | 3215 | 0.0 | 28.623247 | 43 |
CGTATGC | 3210 | 0.0 | 28.527647 | 41 |
TCGTATG | 3210 | 0.0 | 28.457554 | 40 |
CTCGTAT | 3235 | 0.0 | 28.376738 | 39 |
GTATGCC | 3245 | 0.0 | 28.28929 | 42 |
AGGGGCG | 40 | 6.159709E-4 | 28.124615 | 7 |
TCTCGTA | 3290 | 0.0 | 27.902353 | 38 |
GACCTAT | 3260 | 0.0 | 27.814037 | 26 |
CCTATTC | 3255 | 0.0 | 27.787638 | 28 |
AATCTCG | 3275 | 0.0 | 27.755344 | 36 |