FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_30.3510000004171e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_30.3510000004171e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405806
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC205385.061039018644377RNA PCR Primer, Index 13 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT79961.9703996490934093No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG33210.8183713399999013No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA8130.2003420353567961No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT5510.13577916541401555No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC4210.10374415361034584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC20750.044.34879345
GGGGCGA301.1387127E-437.4994858
TTGTCGC250.002105647235.99950819
CCGGGAT352.817409E-432.14241811
GGGCGAA352.817409E-432.1424189
CACCGGG352.817409E-432.1424189
ATGCCGT28850.031.89730844
TACCCAG300.00514372629.999595
ACCGCGA453.4856646E-529.9995888
TACCGCG453.4856646E-529.9995887
TATGCCG32150.028.62324743
CGTATGC32100.028.52764741
TCGTATG32100.028.45755440
CTCGTAT32350.028.37673839
GTATGCC32450.028.2892942
AGGGGCG406.159709E-428.1246157
TCTCGTA32900.027.90235338
GACCTAT32600.027.81403726
CCTATTC32550.027.78763828
AATCTCG32750.027.75534436