FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_29.351000000416f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_29.351000000416f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291233
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC3412811.718452235838658RNA PCR Primer, Index 13 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT102313.5129947499081493No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG58922.0231223796753803No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA23410.8038237425017083No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT15420.5294729649455934No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC15090.5181418314545398No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTGTCTCTTATACA10650.36568658084763744No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA10580.3632830070768079No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT10180.3495482998149248No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT9180.3152115316602171No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA8750.30044672135369277No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCAT7170.2461946276692545No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA6960.2389839063567659No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT6830.23452012649665385No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACACT6120.21014102110681138No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGA6040.20739407965443477No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT5770.1981231522526637No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGG5560.19091243094017504No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATC5230.1795812974491215No Hit
GGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACCT4390.15073841219916698No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCTGTCTCTTATACAC4220.14490116161286667No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATT4140.14215422016049006No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCTGTCTCTT3950.13563023421109557No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGC3720.1277327775355128No Hit
GGGAAACCCAGTGTGTTTCGACACACTATCATTAACCTGTCTCTTATACAC3500.1201786885414771No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCCT3310.11365470259208264No Hit
GGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATAC3290.11296796722898847No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCCTGTCTCTTATAC3220.11056439345815894No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACC3200.10987765809506476No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTCTGTCTCTTATA3160.10850418736887647No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACCTGTCTCTTATA3120.10713071664268814No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACCTG3100.10644398127959401No Hit
GGGAAACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTA3100.10644398127959401No Hit
AACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCC3090.10610061359804693No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGCTGTCTCTT3000.10301030446412322No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT2920.10026336301174663No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG207.009162E-445.02011
TGCCGTC35400.044.80624445
TGAGTAA356.2131476E-638.5886573
GCTGGTC250.00210524135.99752837
CCGTGAC458.6749606E-734.99759734
ATGCCGT45650.034.74569744
ACGTTAT401.5518304E-533.7650761
TAACGCA401.5518304E-533.7650764
GTATAAC401.5518304E-533.7650761
TATGTGC551.1184056E-732.741896
CGGGATA352.816376E-432.1406517
TATCGCC352.816376E-432.1406533
TATGCCG51150.030.96561443
CGTATGC51450.030.82878541
GTATGCC51550.030.81262642
AATCTCG50900.030.80829836
CTCGTAT51300.030.78735739
TCGTATG51350.030.7573840
ATCTCGT51100.030.73174537
AGACCTA51650.030.7094125
Produced by FastQC (version 0.11.2)