FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_27.351000000416a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_27.351000000416a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248536
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC103074.14708533170245RNA PCR Primer, Index 13 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT68542.7577493803714552No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT17630.70935397688866No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA14560.5858306241349343No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT11550.4647214085685776No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG9870.397125567322239No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC5070.2039945923327003No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT4650.18709563202111565No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4310.17341552129269No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC4130.1661731097305823No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT4080.16416132874110792No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC4020.16174719155373868No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA3900.15691891717900022No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATACAC3550.1428364502526797No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT3510.1412270254611002No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT3390.13639875108636174No Hit
AACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATAC3350.13478932629478224No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT3320.13358225770109763No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA3290.132375189107413No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGACTGTCTCTTATAC3200.12875398332635918No Hit
TCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATAC3120.1255351337432002No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC3000.1207068593684617No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGA2880.11587858499372325No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA2820.11346444780635401No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA2790.11225737921266939No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC2600.10461261145266681No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA2570.1034055428589822No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCT2500.10058904947371809No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT207.0226047E-445.0000047
TGCCGTC11350.044.40528545
TGTCCCC401.5556678E-533.75000445
AGCCGCG352.8140598E-432.1428619
CGTGCCA352.8140598E-432.1428610
AACTCCG352.8140598E-432.142865
ACTCCGT352.8140598E-432.142866
GCAGCCG352.8140598E-432.1428617
CCGCTGA702.8670911E-828.92857426
CAGCCGC406.1524403E-428.12500218
CCGTGCC406.1524403E-428.1250029
TATGCAG406.1524403E-428.1250025
GCCGCGG406.1524403E-428.12500220
TCAATAC406.1524403E-428.1250023
GTGCCAG406.1524403E-428.12500211
ATGCCGT18500.027.24324244
CCCCGCT755.6019417E-827.00000224
TCCCCGC755.6019417E-827.00000223
TAACTCC507.1362236E-527.04
CTGGGTC1105.456968E-1226.5909145
Produced by FastQC (version 0.11.2)