FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_25.3510000004166f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_25.3510000004166f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139250
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC15591.1195691202872533RNA PCR Primer, Index 13 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT5040.36193895870736087No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT1970.1414721723518851No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC1940.1393177737881508No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT1740.12495511669658886No Hit
GTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGA1690.12136445242369838No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC1660.1192100538599641No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT1630.1170556552962298No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT1610.11561938958707361No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC1570.1127468581687612No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT1550.11131059245960502No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG1510.10843806104129264No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC1440.10341113105924596No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2100.039.63842845
ATAGTGC403.4321238E-739.370627
GCACCCT301.1307282E-437.5227556
TAGAGGT250.002094517736.0218474
GTTAGGC250.002094517736.0218473
AGAGTGC250.002094517736.0218476
TGATAGC250.002101920835.9959825
CACGGAG250.002101920835.9959834
ATCGTTC250.002101920835.9959831
ACGGAGT250.002101920835.9959835
GGAGTTA250.002101920835.9959837
AGTTAAC250.002101920835.9959827
CGGAGTT250.002101920835.9959836
ATGCCGT2500.033.2962844
TATTATT352.7977614E-432.162362
ACCCTCC352.8095892E-432.1392678
AATTGTC352.8095892E-432.13926717
TAGTGCT501.9668478E-631.4964828
AGTGCTG651.3864337E-831.15036429
CGAGACC2700.030.8298923
Produced by FastQC (version 0.11.2)