FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_24.35100000041646.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_24.35100000041646.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124750
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC14211.1390781563126253No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCA5550.44488977955911824No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC5130.4112224448897796No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT3610.28937875751503006No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTC3310.26533066132264527No Hit
GTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTC3170.25410821643286574No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT2940.23567134268537077No Hit
GGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGG2820.22605210420841684No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATG2730.2188376753507014No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGA2730.2188376753507014No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGA2420.19398797595190378No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT2390.19158316633266534No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT2360.18917835671342687No Hit
GCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTG2250.18036072144288579No Hit
CATCCTGGCTGTCTGGGCCTTCCCACATCGTTTCCCACTTAACCATGACTT2180.174749498997996No Hit
GTATCAACGCAGAGTACGGGCATCCTCCAAGGCTAAATACTCCTGACTGAC2150.17234468937875752No Hit
CCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACG2130.17074148296593186No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA2100.1683366733466934No Hit
GTATCAACGCAGAGTACGGGGCGGTCCGGCCGGGAACTCAAAGGAGACTGC2100.1683366733466934No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA2070.1659318637274549No Hit
GTCTGGGCCTTCCCACATCGTTTCCCACTTAACCATGACTTTGGGACCTTA2040.16352705410821644No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTC2010.16112224448897797No Hit
TATCAACGCAGAGTACGGGGCGGTCCGGCCGGGAACTCAAAGGAGACTGCC2000.16032064128256512No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC1980.15871743486973947No Hit
TGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTT1900.1523046092184369No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCC1870.14989979959919839No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGCCC1870.14989979959919839No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTG1860.14909819639278557No Hit
ACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCA1830.1466933867735471No Hit
TATCAACGCAGAGTACGGGCATCCTCCAAGGCTAAATACTCCTGACTGACC1820.14589178356713428No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCG1770.14188376753507015No Hit
GGTATCAACGCAGAGTACGGGGCGGTCCGGCCGGGAACTCAAAGGAGACTG1770.14188376753507015No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCA1750.1402805611222445No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTG1650.13226452905811623No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1620.12985971943887775No Hit
GTCCCAAAGTCATGGTTAAGTGGGAAACGATGTGGGAAGGCCCAGACAGCC1620.12985971943887775No Hit
CTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCG1610.12905811623246494No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCC1600.1282565130260521No Hit
GTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA1590.12745490981963928No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG1580.12665330661322646No Hit
TTACTCACCCGTCCGCCACTCGTCAGCAAAGAAGCAAGCTTCTTCCTGTTA1540.12344689378757515No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC1530.12264529058116233No Hit
CTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCTTGATTTTCCGGAT1500.12024048096192384No Hit
CTTCTAAGCCAACATCCTGGCTGTCTGGGCCTTCCCACATCGTTTCCCACT1490.11943887775551101No Hit
GGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAA1480.11863727454909818No Hit
TCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCACCT1420.11382765531062125No Hit
TTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCC1400.11222444889779559No Hit
GTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATT1370.1098196392785571No Hit
CTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGGGCCTTCC1370.1098196392785571No Hit
ACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGG1350.10821643286573147No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGCTGTCTC1340.10741482965931864No Hit
AGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCC1290.10340681362725451No Hit
GTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAATCCGGAAAATCAAGGCTGA1260.10100200400801602No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTC1250.1002004008016032No Hit
GGTATCAACGCAGAGTACGGGCATCCTCCAAGGCTAAATACTCCTGACTGA1250.1002004008016032No Hit
CTTCACGACGGACGTTAGCACCCGCCGTGTGTCTCCCGTGATAACATTCTC1250.1002004008016032No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATG302.1447759E-645.015642
AGTTGAC206.9971057E-445.015642
TGCCGTC1600.043.59142345
GACCGTT451.9055733E-839.9978647
ACGTTAT301.1317787E-437.513031
CCCACGC301.1317787E-437.513032
TTAGGAC504.8048605E-836.012513
CAGTTGA250.002096350836.012511
GGTATCA5500.035.1940421
ATGCCGT2000.034.87313544
TCTTATC2050.034.0225733
CACGAGA2050.034.0225721
TATGCCG2050.034.0225743
TATCTCG2050.034.0225736
GTGTCTT2050.034.0225730
CCTAGTT401.545799E-533.761732
TAGTTCC401.545799E-533.761734
GACAGTG2100.033.21251326
CTCGTAT2100.033.21251339
CCCACGA2100.033.21251319
Produced by FastQC (version 0.11.2)