FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_23.3510000004162c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_23.3510000004162c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156306
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC147969.466047368623085No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT131588.41810295190204No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCG106026.782849026908757No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC7580.4849461952836104No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA5170.3307614550944942No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT3990.2552685117653833No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA3960.25334919964684655No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCC3500.22391974716261692No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC3430.21944135221936456No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA3120.1996084603278185No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGC2700.17273809066830448No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC2550.1631415300756209No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA2460.15738359372001073No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC2340.14970634524586388No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC2300.14714726242114826No Hit
TGTCTTATGTCTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATC2060.1317927654728545No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT1920.12283597558634986No Hit
TGTCTTATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTT1850.11835758064309752No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC1810.1157984978183819No Hit
TGTCTTATCTCGTACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGT1730.11068033216895065No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG1670.10684170793187721No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC1660.1062019372256983No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG1600.10236331298862489No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAC253.8754668E-544.999689
TGCCGTC14750.043.1691845
GATGTTA501.0695658E-940.499716
TGCTTAG552.7175702E-936.8179218
ACCTGAT552.7175702E-936.8179212
TGCTTGA458.6366344E-734.9997525
GCTTGAA458.6366344E-734.9997526
ACGGGAT458.6366344E-734.999753
CCGTCTT458.6366344E-734.9997517
GAGGCTT551.1172051E-732.7270424
GGCTTTT501.9672843E-631.49977526
TTAGAGG651.3873432E-831.15362421
ATTTGAT651.3873432E-831.1536242
CTTGTTA300.00513429.99978615
CTACTGT300.00513429.99978634
AGAGGCT602.396573E-729.99978623
ATACCCG300.00513429.99978619
TAGAGGC702.852903E-828.92836622
ATGCTTA702.852903E-828.92836617
TACCTGA702.852903E-828.92836611
Produced by FastQC (version 0.11.2)