FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_21.351000000415e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_21.351000000415e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251122
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC109634.365607155087965No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT98413.918812370083067No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCG44501.7720470528269128No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA29011.1552153933148033No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT15440.6148405954078098No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA9430.37551469007096155No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGA6850.27277578228908655No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC6300.25087407714178767No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT6180.2460955232914679No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA5940.23653841559082836No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA5860.23335271302394853No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA5710.2273795207110488No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATACAC4970.19791177196741028No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT4950.1971153463256903No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC4770.18994751555021064No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC4650.1851689616998909No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT4530.18039040784957114No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4460.17760291810355128No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCTCTTATAC4260.16963866168635164No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC4130.16446189501517192No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC3970.15809048988141222No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA3690.14694053089733275No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3550.14136555140529306No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATC3490.13897627448013317No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTGTCTCTTATACA3460.13778163601755322No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTCTGTCTCTTATACACATCTC3400.13539235909239333No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA3110.1238441872874539No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA2970.11826920779541418No Hit
GGGAAACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTA2850.11349065394509442No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTCTGTCTC2810.1118978026616545No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGCTGTCTCTTATACAC2800.11149958984079451No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTCTGTCTCTTATACACAT2780.11070316419907456No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGC2720.10831388727391468No Hit
AAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACACAT2710.1079156744530547No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA2700.10751746163219472No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA2660.10592461034875479No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACC2630.10472997188617486No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA2580.10273890778187493No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC2570.10234069496101496No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC2570.10234069496101496No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC11050.043.9815145
TATACGT356.2291856E-638.5710832
GCTTTAG250.00209996136.0140231
CGAACCC250.00210407235.9996831
ACCGTGT250.00210407235.999688
TTCTCCG250.00210407235.999689
CGTCCAC401.555827E-533.7497026
ATTCAGC606.3901098E-933.749745
CTGAAAC5400.032.4997144
CGTGTTT501.9736126E-631.49972216
TGAAACA5100.030.44090845
GTACAGT300.005129907330.0116881
GTACAGC300.00513991229.9997336
AAGACTA300.00513991229.99973323
CCGTCTT300.00513991229.99973317
CGGGAGC300.00513991229.99973317
AGACAGC300.00513991229.9997336
CAGCTTG453.4803128E-529.9997339
ATTGGAC300.00513991229.99973326
TGTACGG901.2732926E-1129.99973320
Produced by FastQC (version 0.11.2)