FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_15.3510000004153a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_15.3510000004153a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400992
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC243146.063462612720453No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT185664.630017556459979No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT26860.6698387997765541No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG18850.4700841912058096No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC15120.37706487909983244No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC8760.21845822360545847No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT8310.2072360545846301No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT7900.1970114116989865No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA7560.18853243954991622No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA7360.1835448088739925No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT6870.1713251137179794No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT6800.16957944298140612No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA6680.1665868645758519No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT6570.16384366770409386No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6480.16159923389992817No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC6330.15785851089298542No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC6140.15312026175085788No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC5980.1491301572101189No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5690.1418980927300295No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG5630.14040180352725243No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGACTGTCTCTTATAC5400.13466602824994015No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5200.12967839757401645No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC4990.12444138536429655No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC4610.11496488708004149No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCT4310.10748344106615594No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC4300.10723405953235975No Hit
ACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACA4180.10424148112680554No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT4060.1012489027212513No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATCG207.02836E-444.9992529
AAACGGC207.02836E-444.99925239
GTCCAGC207.02836E-444.99925211
TGCCGTC25900.044.65176845
GTACCAG250.002104358736.003891
CGGGCGA250.002104358736.003894
CGTGCAT250.002105647535.99940510
CGGCTTT250.002105647535.99940526
TGTCACA352.817399E-432.14232312
GCTAGAA352.817399E-432.14232328
GCAGACG352.817399E-432.14232332
TAACCTA13850.030.05745
AGCGTGC300.00514372629.9995028
GTCTGCG300.00514372629.99950221
ACCGCAG300.00514372629.99950212
CGTCTGC300.00514372629.99950220
CACGATA300.00514372629.99950210
GACTTCG300.00514372629.9995029
ACGATTT300.00514372629.99950211
ACGGCTT300.00514372629.99950225
Produced by FastQC (version 0.11.2)