FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_14.35100000041510.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_14.35100000041510.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520249
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC72461.392794604122257No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT33850.6506499772224454No Hit
TTGTAGTACTTGGCTGATTCTTTCTCATAACGTGTGAGGTTATTTTTGAAA8400.16146114648946946No Hit
TTGTAATACTTGGCTGATTCTTTCTCATAACGTGTGAGGTTATTTTTGAAA7460.1433928753346955No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG6640.12763119198691397No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8150.043.3382845
GTCGCGC401.560231E-533.745898
GGGCTAG401.560231E-533.7458937
GGTCGCG401.560231E-533.745897
ATGCCGT11400.030.98306844
TATGCCG11600.030.44887543
CCCGCAT300.005140507630.00532
AGCGCCA453.4855297E-530.00211720
CGTATGC11800.029.93279541
TCGTATG11950.029.36880740
GTATGCC12050.029.31178342
CTCGTAT11900.029.30315639
CCCACGA12150.029.2613219
GCCCACG12200.029.141418
CGAGACA12200.029.141423
CACGAGA12250.029.02245321
ACGAGAC12300.028.90447822
CCACGAG12350.028.78745520
AGGTCGC554.163121E-628.6328756
AATCTCG12150.028.32988436
Produced by FastQC (version 0.11.2)