FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_11.351000000414a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_11.351000000414a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342154
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC46951.372189131209923No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC40511.1839697913804894No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA20530.6000222122202283No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC20400.5962227534969633No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC18170.5310474230901875No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC14820.43313829445220575No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT14550.4252471109500401No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG13780.402742624666086No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC13720.40098902833227146No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA9920.2899279271906802No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC9720.28408260607796487No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC9140.26713117485109045No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC8670.25339467023620943No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC8300.2425808261776861No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA7840.2291365876184408No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGT7660.22387579861699705No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT7540.22036860594936786No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA7540.22036860594936786No Hit
GTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGA7530.22007633989373207No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT7490.218907275671189No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA7000.20458623894503647No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT6880.20107904627740728No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT6870.2007867802217715No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG6730.19669505544287078No Hit
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC6330.1850044132174401No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACG6300.1841276150505328No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTG6240.1823740187167182No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACT6220.18178948660544667No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA5820.17009884438001602No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT5720.16717618382365837No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATG5720.16717618382365837No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT5660.16542258748984376No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT5600.16366899115602918No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAA5580.16308445904475763No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTC5530.1616231287665788No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA5400.15782367004331382No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT5400.15782367004331382No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG5250.15343967920877732No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG4930.1440871654284328No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCAT4910.1435026333171613No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCA4770.13941090853826055No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCC4710.13765731220444594No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCAT4480.13093519292482333No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA4470.13064292686918755No Hit
CAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4470.13064292686918755No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTT4420.12918159659100872No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCA4410.12888933053537296No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTAC4320.12625893603465105No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTAC4270.12479760575647224No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGA4180.12216721125575034No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGG4100.11982908281066422No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4100.11982908281066422No Hit
CTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGA3940.11515282592049195No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3920.1145682938092204No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC3910.11427602775358464No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTT3890.11369149564231312No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT3710.1084307066408693No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATAC3680.10755390847396201No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGC3550.10375444975069706No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCT3530.10316991763942551No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAG3530.10316991763942551No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3510.10258538552815398No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTACC3460.10112405524997516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCAAC207.02195E-445.00575
ACTCACG253.885491E-544.99912627
TATGTCG207.027008E-444.99912321
TGCCGTC5550.042.97213445
TCACGCA356.2300114E-638.5763174
ATGCCGT6200.038.10409544
CGGGCAA301.13739814E-437.504754
TATGCCG6350.037.20443
CGTATGC6400.036.91334541
TCGTATG6400.036.91334540
ATCTCGT6500.036.69159337
CTCGTAT6500.036.69159339
GTATGCC6550.036.06842
CGCATAC250.002105246535.999324
GTCGTAT250.002105246535.999324
TCGTATT250.002105246535.999325
TGTCGTA250.002105246535.999323
AAATCTC6700.035.93213335
CCCACGA6700.035.5963219
AATCTCG6700.035.5963236
Produced by FastQC (version 0.11.2)