FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_10.3510000004148b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_10.3510000004148b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65233
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC16452.5217297993346923No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT2560.392439409501326No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC2240.3433844833136603No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC1180.18089004031701744No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA1160.17782410743028837No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG910.13949994634617446No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC900.13796697990280993No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT800.12263731546916438No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC780.11957138258243527No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG690.10577468459215426No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC660.10117578526206061No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCTCG250.002092595436.037
TGCCGTC1950.035.76923445
GACAAAT2150.032.4418632
CATAGAC2150.032.4418628
AGACATA2150.032.4418625
CGAGACA2150.032.4418623
CCCACGA2200.031.70454619
ACATAGA2200.031.70454627
ACGAGAC2200.031.70454622
AAATCTC2150.031.3953535
TCTCGTA2150.031.3953538
AATCTCG2150.031.3953536
TCGTATG2150.031.3953540
ATAGACA2250.031.029
CACGAGA2250.031.021
GAGACAT2250.031.024
GTATGCC2250.031.042
ATGCCGT2250.031.044
CGTATGC2200.030.68181841
ACAAATC2300.030.32608833
Produced by FastQC (version 0.11.2)