FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_1.35100000041362.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_1.35100000041362.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335600
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC69702.0768772348033373No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGT10090.30065554231227654No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC9690.2887365911799762No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA4940.1471990464839094No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC4560.13587604290822408No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC4160.12395709177592373No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC3500.10429082240762813No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG3450.10280095351609059No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8900.036.40425545
TCGTATG9350.034.41153340
CGTTATC9400.034.22849733
TATCTCG9350.034.17089536
AATGCGT9300.034.11267529
GCGTTAT9400.033.98913632
ATGCCGT9500.033.868244
CGTATGC9550.033.69087641
CTCGTAT9500.033.6313639
CACGAGA9500.033.6313621
CCCACGA9500.033.6313619
ATGCGTT9500.033.6313630
GTATGCC9700.033.4018442
CGAGACA9500.033.39451623
GTTATCT9650.033.34174734
TATGCCG9700.033.16988443
TGCGTTA9650.033.10858531
AGACAAT9600.033.04665425
ATCTCGT9700.032.93792737
GAGACAA9700.032.93792724