FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_96.3510000004732c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_96.3510000004732c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTC1113.253012048192772No Hit
ATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTCTTC22.4096385542168677RNA PCR Primer, Index 45 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTCT22.4096385542168677No Hit
GCTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCAC11.2048192771084338No Hit
TTATACACATCTCCGAGCCCACGAGAACTTCCTTCATCTCGTATGCCGTCT11.2048192771084338RNA PCR Primer, Index 45 (95% over 22bp)
AACGCAGAGTACGGGGTGGTAACAAACGCAAAAAAAAAAAAAAAAAAAAAA11.2048192771084338No Hit
GTCTGGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCCCTGGAAAGG11.2048192771084338No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.2048192771084338No Hit
TATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTCTT11.2048192771084338RNA PCR Primer, Index 7 (95% over 21bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACCACCCCCGTACTCT11.2048192771084338No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTACCCCCCCTT11.2048192771084338No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTTTATCCCC11.2048192771084338No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTGACACCCCCTCCC11.2048192771084338No Hit
ATTTAAGATTTTTCTTTTCCTTATCGGCTTTTGTTTCTTTCAAGATTTTTT11.2048192771084338No Hit
CTGCAACCTCCACCTCCCAGGTTCAAGTGATTCTCCTGCTTCAGCCTGCCG11.2048192771084338No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGATTTATCTCGTATGCCGTC11.2048192771084338TruSeq Adapter, Index 2 (95% over 21bp)
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA11.2048192771084338No Hit
AGGTACAGAGTTGTCAGTTAGGGTAGATAAAAAATTTTGGAAAAGGGTGGT11.2048192771084338No Hit
GTATAATGGGTCAGCGACTTACATTCAGTGGCGAGGTTAACCGAATAGGGA11.2048192771084338No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCT11.2048192771084338No Hit
GTACGGGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAA11.2048192771084338No Hit
CGGGAGAGTACTTTTTTTTTTTTTTTTTTGGTATCAACGCAAAAAAAAAAA11.2048192771084338No Hit
CTGTCGGCGCTTCATGGCGATCCCAGGCGTGGGTCCGGTGACGGCGTTGAG11.2048192771084338No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAAAAAAAT11.2048192771084338No Hit
AAGCATTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGCC11.2048192771084338No Hit
GTACTCTGCGTTGATACCACTGCTTGATACCACTGCTTCCCGTACTCCTGT11.2048192771084338No Hit
ACGGGAAGTGGTATCAACGCCAAAAAAAAAAAAAAAAAAAAGTATTCTGTG11.2048192771084338No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCATCCCTCCTTTACACCC11.2048192771084338No Hit
GACTGAGGCCGGAGAATCACTTGAACCTGGGAGGCAAAGTTTGCAGTGAGC11.2048192771084338No Hit
CAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGCATGCTCACACTCGAA11.2048192771084338No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAA11.2048192771084338No Hit
GACCCAGAGTCGACGAATCCGCTGGCTCGCAAAGCGGCGCCAGCACCCGCT11.2048192771084338No Hit
GCGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCC11.2048192771084338No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGTAAACCACCCC11.2048192771084338No Hit
GCCTCCTCCAATGTGGAGTGCGTCTCCGATCTTGTTGATGAGTCCTGCCAT11.2048192771084338No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.2048192771084338No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAAGGTCATCTCGTATGCCGTC11.2048192771084338No Hit
GCGGAGGCCACAATCGCAGCCCTGCGCAAGCTTGGCGTGCGCCTGTCGATC11.2048192771084338No Hit
CCGCCTGCTCGCGTAGCGATTGAGTGGCGGGGCCCTGAGGGAACACCTGGC11.2048192771084338No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGT11.2048192771084338No Hit
ACTTGGAACTTAACCTAAAACTGTATTTAGTCCATTAGACCTGTCTCTTAT11.2048192771084338No Hit
GAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACCTGTCTCTT11.2048192771084338No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTGT11.2048192771084338No Hit
GAGTACGGGCAGTGGTATCAACGCAGAGTACCTGTCTCTTATACACATCTC11.2048192771084338No Hit
CCTACACCTAATGGCTTAAGCCGCTCTTCCCATTTTCTCGCTGACCCATCA11.2048192771084338No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC11.2048192771084338No Hit
GTTTGGTCCCTATCTTCCGTGGGCGCTGCAAGATTGAGAGAGCCTGCTCCT11.2048192771084338No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCGCTTCATCTCGTATGCCGTC11.2048192771084338No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGT11.2048192771084338No Hit
GTGCTTCGCCGCCGATACGCCGAATCTGACCGCGCAGAACGTCGGTCTTCC11.2048192771084338No Hit
TAGCCAGACTAAGAACAAACGAGAAAATCTAAATGAAAAAGGCACCCTGTC11.2048192771084338No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCCCGTATGCCGTC11.2048192771084338No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTGT11.2048192771084338No Hit
ATCCTACATTATTATGGATTAACTCTGGTGTAGCAACTTTAAAGACTGTCT11.2048192771084338No Hit
CGGCTAGACGGAAAGACCCCATGAACCTTTACTGTAGCTTTGCATTGGACT11.2048192771084338No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCCTGTCTCTTATACACATCTCC11.2048192771084338No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTATACAT11.2048192771084338No Hit
GCTTTACGAGTTCATTGCTATGGTGACTGTTGTGATGATGGTTCTCTCACA11.2048192771084338No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA11.2048192771084338No Hit
GGTCTCAACGCAGAGTACGGGGCAGTAGCATCAACGCAGAGTACTTTTTGT11.2048192771084338No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATCCCCC11.2048192771084338No Hit
CCTCGATGTCGGCTCATCTCATCCTGGGGCTGTAGCCGGTCCCACGGGTAT11.2048192771084338No Hit
CGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAACTCTTCGTTG11.2048192771084338No Hit
GTACGGGAAGCAGTGGTATCAACTTACTTACTTTTTTTTTTTTTTTTTTTT11.2048192771084338No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTT11.2048192771084338No Hit
GCTTTCAGCGGTTATCTCTTCCGCACTTAGCTACTCGGCGATGCCACTGGC11.2048192771084338No Hit
GGTATCAACGCAGAGTACGGGGCAGTGGCTGTCTCTTATACACATCTCCGA11.2048192771084338No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGCTTCATCTCGTATGCCGTC11.2048192771084338No Hit
ACCTTGATCTTCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCG11.2048192771084338No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.2048192771084338No Hit
AGGTAGTTGGTGGGGTAAAAGCTTACCAAGCCGACGATCTGTAGCTGGTCT11.2048192771084338No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC50.045.017
GTATGCC50.045.042
TTCATCT50.045.034
ATCTCGT50.045.037
ATCTCCG50.045.010
CACATCT50.045.07
GCCCACG50.045.018
TATACAC50.045.03
TGCCGTC50.045.045
CCACGAG50.045.020
TATGCCG50.045.043
CATCTCG50.045.036
CATCTCC50.045.09
GAGCCCA50.045.016
GTTCATC50.045.033
CACGAGA50.045.021
GGTTCAT50.045.032
CCCACGA50.045.019
ACATCTC50.045.08
CTAGGTT50.045.029