FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_95.3510000004731f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_95.3510000004731f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72289
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTC2287031.63690187995407No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGT19152.649089073026325No Hit
TTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTCT7481.034735575260413No Hit
ATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTCTTC4670.6460180663724772TruSeq Adapter, Index 7 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCG4500.6225013487529223No Hit
TATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTCTT1980.2739005934512858RNA PCR Primer, Index 7 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC25400.042.16146545
CCGTCTT451.8872015E-839.9963144
ATGCCGT27500.038.9418644
CGTATGC28200.037.97521641
AGCCCAC28150.037.9627517
CTCGTAT28100.037.95023339
TCGTATG28100.037.95023340
TCTCGTA27950.037.91242238
TATGCCG28200.037.89543543
GTATGCC28200.037.89543542
ATCTCGT28000.037.8447237
CGAGCCC28250.037.8283715
ACGAGAC28250.037.8283722
CCTAGGT27850.037.80620628
CTAGGTT27850.037.80620629
GACCTAG27850.037.80620626
ACCTAGG27800.037.79327827
TCATCTC28050.037.77726435
CACGAGA28350.037.77429621
CCCACGA28350.037.77429619