Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0120_pe_95.3510000004731f.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 72289 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 51 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTC | 22870 | 31.63690187995407 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGT | 1915 | 2.649089073026325 | No Hit |
TTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTCT | 748 | 1.034735575260413 | No Hit |
ATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTCTTC | 467 | 0.6460180663724772 | TruSeq Adapter, Index 7 (95% over 22bp) |
CTCTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCG | 450 | 0.6225013487529223 | No Hit |
TATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTCTT | 198 | 0.2739005934512858 | RNA PCR Primer, Index 7 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 2540 | 0.0 | 42.161465 | 45 |
CCGTCTT | 45 | 1.8872015E-8 | 39.99631 | 44 |
ATGCCGT | 2750 | 0.0 | 38.94186 | 44 |
CGTATGC | 2820 | 0.0 | 37.975216 | 41 |
AGCCCAC | 2815 | 0.0 | 37.96275 | 17 |
CTCGTAT | 2810 | 0.0 | 37.950233 | 39 |
TCGTATG | 2810 | 0.0 | 37.950233 | 40 |
TCTCGTA | 2795 | 0.0 | 37.912422 | 38 |
TATGCCG | 2820 | 0.0 | 37.895435 | 43 |
GTATGCC | 2820 | 0.0 | 37.895435 | 42 |
ATCTCGT | 2800 | 0.0 | 37.84472 | 37 |
CGAGCCC | 2825 | 0.0 | 37.82837 | 15 |
ACGAGAC | 2825 | 0.0 | 37.82837 | 22 |
CCTAGGT | 2785 | 0.0 | 37.806206 | 28 |
CTAGGTT | 2785 | 0.0 | 37.806206 | 29 |
GACCTAG | 2785 | 0.0 | 37.806206 | 26 |
ACCTAGG | 2780 | 0.0 | 37.793278 | 27 |
TCATCTC | 2805 | 0.0 | 37.777264 | 35 |
CACGAGA | 2835 | 0.0 | 37.774296 | 21 |
CCCACGA | 2835 | 0.0 | 37.774296 | 19 |