FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_93.351000000472da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_93.351000000472da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335989
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTC239947.141305221301889No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA28560.8500278282919977No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26300.7827637214313563No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGT24900.7410956906327291No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21010.6253180907708289No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18670.5556729535788374No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18000.53573182455378No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA17880.5321602790567548No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA16690.4967424528779216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15690.466979573736045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14360.427394944477349No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA12940.3851316560958841No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12640.3762027923533211No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11540.3434636252972567No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA11480.34167785254874417No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA10680.3178675492352428No Hit
TTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTCT8740.2601275637000021No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8260.24584138171190126No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8050.2395911770921072No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA7890.2348291164294069No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA7490.22292396477265625No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA6860.20417335091327393No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6710.19970891904199242No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5790.17232707023146593No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCG5490.1633982064889029No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA4910.14613573658661444No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA4840.14405233504668308No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA4700.13988553196682035No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4430.13184955459851364No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA4310.12827800910148845No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT4120.12262306206453188No Hit
ATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTCTTC3940.11726574381899409TruSeq Adapter, Index 7 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT3930.11696811502757531No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT3920.11667048623615654No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA3840.11428945590480641No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3740.11131316799061874No Hit
GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA3660.10893213765926861No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGT207.029014E-444.99627720
TACGACA207.029014E-444.99627741
TGCCGTC25250.041.17104345
AACACTA356.2258896E-638.57972326
GTTAACT301.1377589E-437.50248342
ATCTCGT28700.036.2949137
CCTAGGT28450.036.22923328
CTAGGTT28450.036.22923329
GACCTAG28650.036.21197526
TAGGTTC28600.036.19659430
AGACCTA28800.036.17965325
TATGCCG28800.036.17426743
CGTATGC28750.036.15353441
CACGAGA28950.036.14766721
CTCGTAT28900.036.12158239
ACCTAGG28550.036.10233327
CATCTCG28800.036.09076336
ACGAGAC29000.036.08534622
GTATGCC28850.036.0335842
ATGCCGT28850.036.0335844
Produced by FastQC (version 0.11.2)