FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_91.35100000047297.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_91.35100000047297.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55427
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTC1771331.957349306294763No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGT15522.8000793836938676No Hit
TTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTCT5470.9868836487632381No Hit
ATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTCTTC4180.7541450917422917TruSeq Adapter, Index 7 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCG3490.6296570263589947No Hit
TATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTCTT1570.28325545311851624RNA PCR Primer, Index 7 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC18750.040.3245
ATGCCGT20700.036.5217444
TATGCCG20800.036.34615743
CGTATGC20850.036.25899541
GTATGCC20850.036.25899542
CCGAGCC20850.036.25899514
ATCTCGT20800.036.23798437
CATCTCG20800.036.23798436
TCTCGTA20800.036.23798438
TCGTATG20800.036.23798440
CTCGTAT20850.036.1510839
TCATCTC20850.036.1510835
TTCATCT20800.036.1298134
CGAGCCC20950.036.0859215
TCCGAGC21050.036.02137813
GAGCCCA21000.035.99999616
AGCCCAC20900.035.95693617
CTCCGAG21100.035.93601612
CCTAGGT20850.035.93525328
TAGGTTC20850.035.93525330